环状 RNA circitga7 通过 miR-34a-5p/VEGFA 轴促进神经胶质瘤进展。

Circular RNA circitga7 accelerates glioma progression via miR-34a-5p/VEGFA axis.

机构信息

The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan 511518, Guangdong, China.

Department of Pathophysiology, Jilin Medical University, Jilin 132013, Jilin, China.

出版信息

Aging (Albany NY). 2021 May 7;13(9):13138-13152. doi: 10.18632/aging.202996.

Circular RNAs (circRNAs) are a group of noncoding RNAs derived from back-splicing events. CircRNA is reported to be involved in various tumor progressions, including glioma. Although there are a few reports of circular RNAs participating in gliomas, it is still unclear whether circular RNAs regulate the occurrence of gliomas. In our research, we found that the expression of circITGA7 in glioma tissues and glioma cells increased significantly. Knocking down circITGA7 can significantly inhibit the proliferation of glioma cells and reduce cell metastasis. Through analysis and dual-luciferase report assay, we found that circITGA7 acts as a sponge for miR-34a-5p targeting VEGFA in glioma. Our study showed that circITGA7 regulates the proliferation and metastasis of glioma cell lines (SW1783&U373) by regulating the miR-34a-5p/VEGFA pathway. In conclusion, our study revealed a regulatory loop for the circITGA7/miR-34a-5p/VEGFA axis to regulate glioma development.

环状 RNA（circRNAs）是一类来源于反向剪接事件的非编码 RNA。已有报道称 circRNA 参与多种肿瘤的进展，包括神经胶质瘤。尽管有一些关于环状 RNA 参与神经胶质瘤的报道，但环状 RNA 是否调节神经胶质瘤的发生仍不清楚。在我们的研究中，我们发现 circITGA7 在神经胶质瘤组织和神经胶质瘤细胞中的表达显著增加。敲低 circITGA7 可以显著抑制神经胶质瘤细胞的增殖并减少细胞转移。通过分析和双荧光素酶报告实验，我们发现 circITGA7 作为 miR-34a-5p 的海绵体，靶向 VEGFA 在神经胶质瘤中。我们的研究表明，circITGA7 通过调节 miR-34a-5p/VEGFA 通路来调节神经胶质瘤细胞系（SW1783 和 U373）的增殖和转移。总之，我们的研究揭示了 circITGA7/miR-34a-5p/VEGFA 轴的调节环路，以调节神经胶质瘤的发生。