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从花中分离的DB-21亚种菌株来源的外泌体的美白和抗炎活性

Whitening and Anti-Inflammatory Activities of Exosomes Derived from subsp. DB-21 Strain Isolated from Flower.

作者信息

Choi Byeong-Min, Lee Gibok, Hong Hyehyun, Park Chang-Min, Yeom Areum, Chi Won-Jae, Kim Seung-Young

机构信息

Department of Life Science and Biochemical Engineering, Sunmoon University, Asan 31460, Republic of Korea.

R&D Center, Hankook Cosmetics Manufacturing Co., Ltd., 35 Cheonggyecheon-ro, Jongno-gu, Seoul 03188, Republic of Korea.

出版信息

Molecules. 2025 Feb 28;30(5):1124. doi: 10.3390/molecules30051124.

DOI:10.3390/molecules30051124
PMID:40076347
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11901582/
Abstract

In the present study, we investigated the anti-inflammatory and anti-melanogenic effects of subsp. DB-21-derived exosomes (DB-21 exosomes), isolated from flower in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells and melanocyte-stimulating hormone (α-MSH)-induced B16F10 melanoma cells. We confirmed that DB-21 exosomes were not toxic to LPS-induced RAW 264.7 macrophage cells and α-MSH-induced B16F10 melanoma cells. Moreover, we confirmed that DB-21 exosomes inhibit the pro-inflammatory cytokines IL-6, IL-1β, TNF-α, PGE, and the expression of inflammatory factors iNOS and COX-2. We also found that DB-21 exosomes have a concentration-dependent ability to inhibit melanin, TRP-1, TRP-2, tyrosinase, and MITF, which are factors involved in melanogenesis. Additionally, it inhibits the phosphorylation of Akt and GSK-3β, and MAP kinase pathway proteins such as ERK, JNK, and p38. We confirmed that DB-21 exosomes inhibit melanin synthesis in B16F10 cells through various pathways, and based on previous results, they may be used as a functional cosmetic material with anti-inflammatory and anti-melanogenic activities.

摘要

在本研究中，我们研究了从[花名]亚种DB - 21中分离出的外泌体（DB - 21外泌体）在脂多糖（LPS）诱导的RAW 264.7巨噬细胞和促黑素细胞激素（α - MSH）诱导的B16F10黑色素瘤细胞中的抗炎和抗黑素生成作用。我们证实DB - 21外泌体对LPS诱导的RAW 264.7巨噬细胞和α - MSH诱导的B16F10黑色素瘤细胞无毒。此外，我们证实DB - 21外泌体可抑制促炎细胞因子IL - 6、IL - 1β、TNF - α、PGE以及炎症因子iNOS和COX - 2的表达。我们还发现DB - 21外泌体具有浓度依赖性抑制黑色素、TRP - 1、TRP - 2、酪氨酸酶和MITF的能力，这些都是参与黑素生成的因子。此外，它还抑制Akt和GSK - 3β以及MAP激酶途径蛋白如ERK、JNK和p38的磷酸化。我们证实DB - 21外泌体通过多种途径抑制B16F10细胞中的黑色素合成，基于先前的结果，它们可用作具有抗炎和抗黑素生成活性的功能性化妆品原料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/7e5186406224/molecules-30-01124-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/ec2f1b8010f1/molecules-30-01124-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/eed3f46b0687/molecules-30-01124-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/569227cb62f8/molecules-30-01124-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/31d044806f79/molecules-30-01124-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/7d1a4e0ab245/molecules-30-01124-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/e1e9c971545c/molecules-30-01124-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/08817ab970bd/molecules-30-01124-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/312d7aaea769/molecules-30-01124-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/9eecd2104ba3/molecules-30-01124-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/7e5186406224/molecules-30-01124-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/ec2f1b8010f1/molecules-30-01124-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/eed3f46b0687/molecules-30-01124-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/9e42e7b71d19/molecules-30-01124-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/569227cb62f8/molecules-30-01124-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/31d044806f79/molecules-30-01124-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/7d1a4e0ab245/molecules-30-01124-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/e1e9c971545c/molecules-30-01124-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/08817ab970bd/molecules-30-01124-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/312d7aaea769/molecules-30-01124-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/9eecd2104ba3/molecules-30-01124-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25cd/11901582/7e5186406224/molecules-30-01124-g011.jpg

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