Ueda T, Ishida Y
J Chromatogr. 1987 Jan 16;386:273-82. doi: 10.1016/s0021-9673(01)94603-5.
The fundamental characteristics of a new series of ion-exchange columns, specifically designed for high-speed analysis of biological macromolecules, have been examined. Each of these columns is packed with a chemically modified silica gel, that has a controlled pore-size distribution and a uniform particle diameter. Surface silanol groups of these materials are chemically modified with hydrophilic polymers to produce a neutral silica surface, and ionic functional groups are chemically bonded to this neutral support, producing a stable ion exchanger. The separation of biological macromolecules on these columns has been investigated, and the selection of a mobile phase is discussed.
已对专门设计用于生物大分子高速分析的一系列新型离子交换柱的基本特性进行了研究。这些柱子中的每一根都填充有化学改性硅胶，其具有可控的孔径分布和均匀的粒径。这些材料的表面硅醇基团用亲水性聚合物进行化学改性，以产生中性硅胶表面，并且离子官能团化学键合到这种中性载体上，从而产生稳定的离子交换剂。已经研究了这些柱子上生物大分子的分离，并讨论了流动相的选择。
