Rezaian M A, Krake L R
CSIRO, Division of Horticultural Research, Adelaide, S.A., Australia.
J Virol Methods. 1987 Sep;17(3-4):277-85. doi: 10.1016/0166-0934(87)90137-6.
Three extraction media for the isolation of nucleic acids from grapevines, a tissue high in polyphenols and other materials that interfere with nucleic acid extraction, were compared. When phenol was present in the initial extraction media only a small yield of soluble RNA and no high molecular weight rRNA was obtained. In the absence of phenol in conventional salt and detergent-based extraction media, rRNAs were extracted, but a major proportion of the RNAs were broken down. Using Na-perchlorate, a chaotropic salt, in a rapid procedure, it was possible to extract both high and low molecular weight RNA efficiently. This procedure enabled the detection of viral RNA, which could not be detected following phenol extraction, at the picogram levels, by dot-blot hybridization.
比较了三种用于从葡萄藤中分离核酸的提取介质，葡萄藤组织富含多酚和其他干扰核酸提取的物质。当初始提取介质中存在苯酚时，仅获得少量可溶性RNA，未获得高分子量rRNA。在传统的基于盐和洗涤剂的提取介质中不存在苯酚的情况下，可以提取rRNA，但大部分RNA被分解。使用高氯酸钠（一种离液盐），通过快速程序可以高效提取高分子量和低分子量RNA。该程序能够通过斑点印迹杂交在皮克水平检测到苯酚提取后无法检测到的病毒RNA。
