Maule A J, Hull R, Donson J
J Virol Methods. 1983 Apr;6(4):215-24. doi: 10.1016/0166-0934(83)90048-4.
A solid-phase nucleic acid hybridization technique for the detection of DNA and RNA viruses in plant tissues is described. The method involves spotting crude samples onto nitrocellulose and using 12P-labelled DNA hybridization probes. The limit of sensitivity is 5-20 pg virus/spot or approximately 5 micrograms/g leaf tissue. The method is quantitative for DNA viruses in crude homogenates, but not for RNA viruses. The amount of cauliflower mosaic virus in infected leaves and protoplasts was estimated. The amplitude of spot hybridization to screening plant material from glasshouses and field is discussed.
本文描述了一种用于检测植物组织中DNA和RNA病毒的固相核酸杂交技术。该方法包括将粗样品点样到硝酸纤维素膜上，并使用³²P标记的DNA杂交探针。灵敏度极限为5 - 20 pg病毒/斑点或约5微克/克叶片组织。该方法对粗匀浆中的DNA病毒是定量的，但对RNA病毒不是。估计了感染叶片和原生质体中花椰菜花叶病毒的含量。讨论了斑点杂交在筛选温室和田间植物材料中的应用范围。
