Yamada Riko, Tsunashima Tadasuke, Takei Mitsuki, Sato Tatsunori, Wajima Yoshiki, Kawase Makoto, Oshikiri Shotaro, Kajitani Yusuke, Kosoba Keita, Ueda Hiroyuki, Abe Koko, Itoi Shiro, Sugita Haruo
Department of Marine Science and Resources, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
The Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo 108-8477, Japan.
Mar Drugs. 2017 Feb 25;15(3):56. doi: 10.3390/md15030056.
Tetrodotoxin (TTX) is a potent neurotoxin that acts specifically on voltage-gated sodium channels on excitable membranes of muscle and nerve tissues. The biosynthetic process for TTX is unclear, although marine bacteria are generally thought to be the primary producers. The marine flatworm is a known TTX-bearing organism, and is suspected to be a TTX supplier to pufferfish. In this study, flatworm specimens were collected from an intertidal zone in Hayama, Kanagawa, Japan, the TTX content of the flatworm was measured using liquid chromatography with tandem mass spectrometry (LC-MS/MS), and seasonal changes in TTX content were investigated. No significant difference in TTX concentration of the flatworm body was found between the spawning period and other periods. However, the TTX content in individual flatworms was significantly higher in the spawning period than at other times. The TTX content rose in association with an increase in the body weight of the flatworm.
河豚毒素（TTX）是一种强效神经毒素，它特异性作用于肌肉和神经组织可兴奋膜上的电压门控钠通道。尽管一般认为海洋细菌是河豚毒素的主要生产者，但河豚毒素的生物合成过程尚不清楚。海扁虫是一种已知的携带河豚毒素的生物，并且被怀疑是河豚的河豚毒素供应者。在本研究中，从日本神奈川县叶山的潮间带采集了扁虫标本，使用液相色谱串联质谱法（LC-MS/MS）测量了扁虫的河豚毒素含量，并研究了河豚毒素含量的季节性变化。在产卵期和其他时期之间，未发现扁虫体内河豚毒素浓度有显著差异。然而，在产卵期，单个扁虫体内的河豚毒素含量明显高于其他时期。河豚毒素含量随着扁虫体重的增加而上升。
