Bell Amy M, Henshall John M, Porto-Neto Laercio R, Dominik Sonja, McCulloch Russell, Kijas James, Lehnert Sigrid A
CSIRO Agriculture, F D McMaster Laboratory Chiswick, Armidale, NSW, 2350, Australia.
CSIRO AgricultureQueensland Bioscience Precinct, Brisbane, QLD, 4067, Australia.
Genet Sel Evol. 2017 Feb 28;49(1):28. doi: 10.1186/s12711-017-0303-8.
DNA-based predictions for hard-to-measure production traits hold great promise for selective breeding programs. DNA pooling might provide a cheap genomic approach to use phenotype data from commercial flocks which are commonly group-mated with parentage unknown. This study on sheep explores if genomic breeding values for stud sires can be estimated from genomic relationships that were obtained from pooled DNA in combination with phenotypes from commercial progeny.
Phenotypes used in this study were categorical data. Blood was pooled strategically aiming at even pool sizes and within sex and phenotype category. A hybrid genomic relationship matrix was constructed relating pools to sires. This matrix was used to determine the contribution of sires to each of the pools and therefore phenotype category by using a simple regression approach. Genomic breeding values were also estimated using the hybrid genomic relationship matrix.
We demonstrated that, using pooled DNA, the genetic performance of sires can be illustrated as their contribution to phenotype categories and can be expressed as a regression coefficient. Genomic estimated breeding values for sires were equivalent to the regression coefficients and are a commonly used industry tool.
Genotyping of DNA from pooled biological samples offers a cheap method to link phenotypic information from commercial production animals to the breeding population and can be turned into information on the genetic value of stud sires for traits that cannot be measured in the stud environment.
基于DNA对难以测量的生产性状进行预测，对选择性育种计划具有巨大潜力。DNA混合池可能提供一种廉价的基因组方法，以利用来自商业羊群的表型数据，这些羊群通常是群体交配且系谱未知。本项关于绵羊的研究探讨了能否根据混合DNA获得的基因组关系以及商业后代的表型来估计种公羊的基因组育种值。
本研究中使用的表型为分类数据。战略性地采集血液样本以确保混合池大小均匀，并按性别和表型类别进行划分。构建了一个将混合池与种公羊关联起来的混合基因组关系矩阵。该矩阵通过简单回归方法用于确定种公羊对每个混合池的贡献，进而确定对表型类别的贡献。还使用混合基因组关系矩阵估计了基因组育种值。
我们证明，使用混合DNA，种公羊的遗传性能可以通过它们对表型类别的贡献来体现，并可以表示为回归系数。种公羊的基因组估计育种值等同于回归系数，是行业常用工具。
对混合生物样本的DNA进行基因分型提供了一种廉价方法，可将商业生产动物的表型信息与育种群体联系起来，并可转化为种公羊在种畜环境中无法测量的性状的遗传价值信息。
