Mo Xiao-Qiang, Wei Hong-Yu, Huang Gan-Rong, Xu Ling-Yuan, Chen Yu-Li, Qi Jiang, Xian Wei, Qin Yan-Chun, Wei Lian-Deng, Zhao Li-Juan, Huang Yan-Qiang, Xing Wei, Pu Hong-Qin, Wei Peng-Ya, Li Chao-Gan, Liang Qiu-Chun
Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China.
World J Gastroenterol. 2017 Feb 14;23(6):1010-1017. doi: 10.3748/wjg.v23.i6.1010.
To explore the induction effects and mechanism of Thumb (ST) on human hepatocellular carcinoma SMMC-7721 cells through the mitochondrial pathway.
The experiments were conducted on three groups: an experimental group (with ST ethanol extracts' concentration being 2.5, 5 and 10 mg/L), a negative control group (with only nutrient solution, 0 mg/L ST ethanol extracts), and a positive control group (2.5 mg/L DDP). The inhibition rate of cell proliferation was checked by using the methyl thiazolyl tetrazolium method, and cell apoptosis was tested by TUNEL method. Furthermore, RT-PCR was used to examine mRNA expression of Fas, FasL, caspase-8, caspase-3, p53 and Bcl-2 genes.
Compared with the negative control group, the inhibition and apoptosis rates of the experimental group with different concentrations of ST extracts on human hepatocellular carcinoma SMMC-7721 cells significantly increased ( < 0.05). Besides, the mRNA expression of FasL and Bcl-2 significantly decreased ( < 0.05) while the mRNA expression of Fas, caspase-8, caspase-3 and p53 increased significantly. When compared with the positive control group, the experimental groups with 5 mg/L ST ethanol extracts showed effects similar to the positive control group.
ST ethanol extracts induced the apoptosis of hepatocellular carcinoma SMMC-7721 cells through up-regulated Fas, caspase-8, caspse-3 and p53, and down-regulated FasL and Bcl-2 in the mitochondrial pathway.
通过线粒体途径探讨山柰（ST）对人肝癌SMMC - 7721细胞的诱导作用及机制。
实验分为三组：实验组（ST乙醇提取物浓度分别为2.5、5和10 mg/L）、阴性对照组（仅含营养液，ST乙醇提取物浓度为0 mg/L）和阳性对照组（2.5 mg/L顺铂）。采用甲基噻唑基四氮唑法检测细胞增殖抑制率，TUNEL法检测细胞凋亡情况。此外，运用RT - PCR检测Fas、FasL、caspase - 8、caspase - 3、p53和Bcl - 2基因的mRNA表达。
与阴性对照组相比，不同浓度ST提取物实验组对人肝癌SMMC - 7721细胞的抑制率和凋亡率显著升高（<0.05）。此外，FasL和Bcl - 2的mRNA表达显著降低（<0.05），而Fas、caspase - 8、caspase - 3和p53的mRNA表达显著升高。与阳性对照组相比，5 mg/L ST乙醇提取物实验组的效果与阳性对照组相似。
ST乙醇提取物通过上调线粒体途径中的Fas、caspase - 8、caspase - 3和p53，下调FasL和Bcl - 2，诱导肝癌SMMC - 7721细胞凋亡。
