提取物通过 ROS-线粒体轴激活 p38 MAPK 信号通路提升宫颈癌细胞凋亡。
Extract Elevates the Cervical Cancer Cell Apoptosis Through ROS-Mitochondrial Axis Activated p38 MAPK Signaling Pathway.
机构信息
College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Heilongjiang, Daqing, P.R. China.
Library and Information Center, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.
出版信息
In Vivo. 2020 Jul-Aug;34(4):1823-1833. doi: 10.21873/invivo.11977.
BACKGROUND/AIM: Picrasma quassioides (P. quassioides) is used in traditional Asian medicine widely for the treatment of anemopyretic cold, eczema, nausea, loss of appetite, diabetes mellitus, hypertension etc. In this study we aimed to understand the effect of P. quassioides ethanol extract on SiHa cervical cancer cell apoptosis.
MATERIALS AND METHODS
The P. quassioides extract-induced apoptosis was analyzed using the MTT assay, fluorescence microscopy, flow cytometry and western blotting.
RESULTS
P. quassioides extract induced cellular apoptosis by increasing the accumulation of cellular and mitochondrial reactive oxygen species (ROS) levels and inhibiting ATP synthesis. Pretreatment with N-Acetylcysteine (NAC), a classic antioxidant, decreased the intracellular ROS production and inhibited apoptosis. In addition, the P38 MAPK signaling pathway is a key in the apoptosis of SiHa cells induced by the P. quassioides extract.
CONCLUSION
The P. quassioides extract exerts its anti-cancer properties on SiHa cells through ROS-mitochondria axis and P38 MAPK signaling. Our data provide a new insight for P. quassioides as a therapeutic strategy for cervical cancer treatment.
背景/目的：毕氏大风子（P. quassioides）在传统亚洲医学中被广泛用于治疗感冒发热、湿疹、恶心、食欲不振、糖尿病、高血压等疾病。在本研究中，我们旨在了解毕氏大风子乙醇提取物对 SiHa 宫颈癌细胞凋亡的影响。
材料和方法
采用 MTT 法、荧光显微镜、流式细胞术和 Western blot 分析毕氏大风子提取物诱导的细胞凋亡。
结果
毕氏大风子提取物通过增加细胞和线粒体活性氧（ROS）水平的积累并抑制 ATP 合成来诱导细胞凋亡。用 N-乙酰半胱氨酸（NAC）预处理，一种经典的抗氧化剂，可减少细胞内 ROS 的产生并抑制细胞凋亡。此外，P38 MAPK 信号通路是毕氏大风子提取物诱导 SiHa 细胞凋亡的关键。
结论
毕氏大风子提取物通过 ROS-线粒体轴和 P38 MAPK 信号通路发挥对 SiHa 细胞的抗癌作用。我们的数据为毕氏大风子作为宫颈癌治疗的一种治疗策略提供了新的见解。
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