Leelatanawit Rungnapa, Uawisetwathana Umaporn, Klanchui Amornpan, Khudet Jutatip, Phomklad Suwanchai, Wongtriphop Somjai, Jiravanichpaisal Pikul, Karoonuthaisiri Nitsara
Microarray Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Khlong Luang, Pathum Thani, Thailand.
Shrimp Genetic Improvement Center (SGIC), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Surat Thani, Thailand.
Mar Biotechnol (NY). 2017 Apr;19(2):125-135. doi: 10.1007/s10126-017-9738-8. Epub 2017 Feb 28.
To reveal molecular mechanism of how polychaetes enhanced reproductive maturation in the male black tiger shrimp (Penaeus monodon), transcriptomic profiles of male reproductive organs (testes and vas deferens) between polychaete-fed and commercial pellet-fed male brooders were compared using cDNA microarray. The overall profiles were distinguishingly different between the two feed groups as well as between testes and vas deferens. Additionally, six of 11 differentially expressed gene identified by the microarray (HNRPUL1 and GCP4 in testes, MAT2B, CDC16, and CSN5 in vas deferens, and SLD5 in both organs) were validated by quantitative real-time PCR (qPCR) and found to exhibit significantly higher expression levels in polychaete-fed shrimp than those in commercial pellet-fed shrimp. From microarray and qPCR results, the differentially expressed transcripts in both testes and vas deferens between different feeds belonged to DNA replication and microtubule nucleation pathways. Interestingly, while the transcripts involved in nutrient uptake and nucleotide biosynthesis were increased only in testes, those involved in protein refolding and apoptosis were increased only in vas deferens. These findings suggest that polychaetes may enhance spermatogenesis by increasing spermatogonia proliferation in testes and by regulating mature spermatozoa in vas deferens.
为揭示多毛类动物如何促进雄性黑虎虾（斑节对虾）生殖成熟的分子机制，使用cDNA微阵列比较了投喂多毛类动物的雄性亲虾和投喂商业颗粒饲料的雄性亲虾的雄性生殖器官（睾丸和输精管）的转录组图谱。两个饲料组之间以及睾丸和输精管之间的总体图谱明显不同。此外，通过微阵列鉴定的11个差异表达基因中的6个（睾丸中的HNRPUL1和GCP4、输精管中的MAT2B、CDC16和CSN5以及两个器官中的SLD5）通过定量实时PCR（qPCR）得到验证，并且发现在投喂多毛类动物的虾中表达水平显著高于投喂商业颗粒饲料的虾。根据微阵列和qPCR结果，不同饲料组之间睾丸和输精管中差异表达的转录本属于DNA复制和微管成核途径。有趣的是，虽然参与营养吸收和核苷酸生物合成的转录本仅在睾丸中增加，但参与蛋白质重折叠和细胞凋亡的转录本仅在输精管中增加。这些发现表明，多毛类动物可能通过增加睾丸中精原细胞的增殖以及调节输精管中的成熟精子来促进精子发生。
