Uengwetwanit Tanaporn, Ponza Pattareeya, Sangsrakru Duangjai, Wichadakul Duangdao, Ingsriswang Supawadee, Leelatanawit Rungnapa, Klinbunga Sirawut, Tangphatsornruang Sithichoke, Karoonuthaisiri Nitsara
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Khlong Nueung, Khlong Luang, Pathum Thani 12120, Thailand.
Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand.
Mar Genomics. 2018 Feb;37:69-73. doi: 10.1016/j.margen.2017.08.007. Epub 2017 Sep 9.
The black tiger shrimp (Penaeus monodon) is an aquatic animal with considerable economic importance. Poor reproductive maturation in captivity impedes sustainable aquaculture production of this species. This study aims to provide transcriptomic information on reproductive organs using 454 pyrosequencing technology. The transcriptome analysis of ovaries and testes revealed 41,136 transcripts with 20,192 contigs. We found novel sets of transcripts completing several important reproductive pathways such as gonadotropin-releasing hormone (GnRH) signaling and progesterone-mediated oocyte maturation. In addition, we found transcripts encoding for receptors crucial for initiation of the maturation process, such as GnRH receptor (GnRHR), voltage-dependent calcium channel L type alpha-1C (CACNA1C) and epidermal growth factor receptor (EGFR). Moreover, we found a putative novel vigillin encoding for an estrogen-induced polysome-associated protein, which has not been reported in penaeid shrimp. These results suggest that the regulatory mechanism of the pathways important to reproductive maturation might be similar to those in the vertebrate. The obtained data will consequently accelerate the study of reproductive biology of this important species to ensure a sustainable shrimp farming industry.
黑虎虾（斑节对虾）是一种具有重要经济价值的水生动物。圈养条件下繁殖成熟度差阻碍了该物种的可持续水产养殖生产。本研究旨在利用454焦磷酸测序技术提供有关生殖器官的转录组信息。卵巢和精巢的转录组分析揭示了41,136个转录本，其中有20,192个重叠群。我们发现了完成若干重要生殖途径（如促性腺激素释放激素（GnRH）信号传导和孕酮介导的卵母细胞成熟）的新转录本组。此外，我们发现了编码对成熟过程起始至关重要的受体的转录本，如GnRH受体（GnRHR）、电压依赖性钙通道L型α-1C（CACNA1C）和表皮生长因子受体（EGFR）。而且，我们发现了一种推测的新型维吉蛋白，其编码一种雌激素诱导的多核糖体相关蛋白，这在对虾中尚未见报道。这些结果表明，对生殖成熟重要的途径的调控机制可能与脊椎动物中的类似。因此，所获得的数据将加速对这一重要物种生殖生物学的研究，以确保虾类养殖业的可持续发展。
