Rebai Olfa, Belkhir Manel, Boujelben Adnen, Fattouch Sami, Amri Mohamed
Research Unit of Functional Neurophysiology and Pathology, 00/UR/08-01, Faculty of Science of Tunis, University El Manar, 2092, Tunis, Tunisia.
Laboratory LIP-MB, National Institute of Applied Sciences and Technology (INSAT), University of Carthage, Tunis, Tunisia.
Environ Sci Pollut Res Int. 2017 Apr;24(10):9605-9613. doi: 10.1007/s11356-017-8584-6. Epub 2017 Feb 28.
Recent studies demonstrate that glyphosate exposure is associated with oxidative stress and some neurological disorders such as Parkinson's pathology. Therefore, phytochemicals, in particular phenolic compounds, have attracted increasing attention as potential agents for neuroprotection. In the present study, we investigate the impact of glyphosate on the rat brain following i.p. injection and the possible molecular target of neuroprotective activity of the phenolic fraction from Morus alba leaf extract (MALE) and its ability to reduce oxidative damage in the brain. Wistar rats from 180 to 240 g were i.p. treated with a single dose of glyphosate (100 mg kg b.w.) or MALE (100 μg mL kg b.w.) for 2 weeks. Brain homogenates were used to evaluate neurotoxicity induced by the pesticide. For this, biochemical parameters were measured. Data shows that MALE regulated oxidative stress and counteracted glyphosate-induced deleterious effects and oxidative damage in the brain, as it abrogated LDH, protein carbonyls, and malonyldialdehyde. MALE also appears to be able to scavenge HO levels, maintain iron and Ca homeostasis, and increase SOD activity. Thus, in vivo results showed that mulberry leaf extract is a potent protector against glyphosate-induced toxicity, and its protective effect could result from synergism or antagonism between the various bioactive phenolic compounds in the acetonic fraction from M. alba leaf extract.
最近的研究表明，接触草甘膦与氧化应激以及一些神经疾病如帕金森氏病理相关。因此，植物化学物质，特别是酚类化合物，作为潜在的神经保护剂已引起越来越多的关注。在本研究中，我们研究了腹腔注射草甘膦后对大鼠脑的影响，以及桑叶提取物（MALE）酚类组分神经保护活性的可能分子靶点及其减少脑氧化损伤的能力。体重180至240克的Wistar大鼠腹腔注射单剂量草甘膦（100毫克/千克体重）或MALE（100微克/毫升/千克体重），持续2周。用脑匀浆评估农药诱导的神经毒性。为此，测量了生化参数。数据表明，MALE调节氧化应激，抵消草甘膦诱导的脑有害影响和氧化损伤，因为它消除了乳酸脱氢酶、蛋白质羰基和丙二醛。MALE似乎还能够清除羟基自由基水平，维持铁和钙的稳态，并增加超氧化物歧化酶活性。因此，体内结果表明，桑叶提取物是对抗草甘膦诱导毒性的有效保护剂，其保护作用可能源于白桑叶提取物丙酮组分中各种生物活性酚类化合物之间的协同作用或拮抗作用。
