Low Marcela, Eisner Christine, Rossi Fabio
Biomedical Research Centre, University of British Columbia, Vancouver, 2222 Health Sciences Mall, BC, Canada, V6T 1Z3.
Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada.
Methods Mol Biol. 2017;1556:179-189. doi: 10.1007/978-1-4939-6771-1_9.
Fibro/adipogenic progenitors (FAPs ) are tissue-resident mesenchymal stromal cells (MSCs). Current literature supports a role for these cells in the homeostasis and repair of multiple tissues suggesting that FAPs may have extensive therapeutic potential in the treatment of numerous diseases. In this context, it is crucial to establish efficient and reproducible procedures to purify FAP populations from various tissues. Here, we describe a protocol for the isolation and cell culture of FAPs from murine skeletal muscle using fluorescence -activated cell sorting (FACS), which is particularly useful for experiments where high cell purity is an essential requirement. Identification, isolation, and cell culture of FAPs represent powerful tools that will help us to understand the role of these cells in different conditions and facilitate the development of safe and effective new treatments for diseases.
成纤维/脂肪生成祖细胞（FAPs）是组织驻留间充质基质细胞（MSCs）。当前文献支持这些细胞在多种组织的稳态和修复中发挥作用，这表明FAPs在众多疾病的治疗中可能具有广泛的治疗潜力。在这种情况下，建立从各种组织中纯化FAP群体的高效且可重复的程序至关重要。在此，我们描述了一种使用荧光激活细胞分选（FACS）从鼠骨骼肌中分离和培养FAPs的方案，这对于高细胞纯度是基本要求的实验特别有用。FAPs的鉴定、分离和细胞培养是强大的工具，将有助于我们了解这些细胞在不同条件下的作用，并促进开发安全有效的疾病新疗法。
