Saclier Marielle, Theret Marine, Mounier Rémi, Chazaud Bénédicte
Department of Biosciences, University of Milan, 20133, Milan, Italy.
Institut NeuroMyoGène, INMG, Université Claude Bernard Lyon 1, INSERM U1217, CNRS UMR5310, Villeurbanne, France.
Methods Mol Biol. 2017;1556:317-327. doi: 10.1007/978-1-4939-6771-1_17.
Skeletal muscle is a highly plastic tissue, which is able to regenerate after an injury. Effective and complete regeneration requires interactions between myogenic precursor cells and several cell types such as macrophages . Bone marrow derived macrophages in mouse and monocyte-derived macrophages in human are useful tools to obtain macrophage populations that may be specifically activated/polarized in vitro (e.g., pro-inflammatory, anti-inflammatory, and alternatively activated macrophages ). In vitro , human or murine primary myogenic cells recapitulate the adult myogenesis program through proliferation, myogenic differentiation, and fusion. Macrophages being highly secreting cells, they act on various biological processes including adult myogenesis . Here, we present protocols to analyze in vitro the effect of macrophage-secreted factors on muscle cell proliferation or differentiation in both mouse and human.
骨骼肌是一种高度可塑性的组织，能够在损伤后再生。有效的完全再生需要成肌前体细胞与多种细胞类型（如巨噬细胞）之间的相互作用。小鼠中的骨髓来源巨噬细胞和人类中的单核细胞来源巨噬细胞是获得可在体外特异性激活/极化的巨噬细胞群体（如促炎性、抗炎性和替代性激活巨噬细胞）的有用工具。在体外，人类或小鼠原代成肌细胞通过增殖、成肌分化和融合来重现成年肌生成程序。巨噬细胞是高度分泌性细胞，它们作用于包括成年肌生成在内的各种生物学过程。在这里，我们展示了在体外分析巨噬细胞分泌因子对小鼠和人类肌肉细胞增殖或分化影响的方案。
