Arquint M, Spencer J H
Department of Biochemistry, Queen's University, Kingston, Ontario, Canada.
J Virol. 1987 Dec;61(12):3694-700. doi: 10.1128/JVI.61.12.3694-3700.1987.
The region of the bacteriophage S13 genome which contains the B, K, and C genes and most of the A, D, and E genes (map positions 627 to 2198) was analyzed for Escherichia coli RNA polymerase-binding sites by combined DNase I footprinting, exonuclease III analysis, and DNA sequencing. Two high-affinity binding sites that correspond to the B and D gene promoters were mapped at positions 936 to 995 and 1779 to 1848, respectively. Positions 936 to 995 are in gene A, preceding the B gene, and positions 1779 to 1848 are in gene C, just preceding the D gene. In addition, two lower-affinity binding sites were identified at positions 1527 to 1538 and 1705 to 1756 preceding the C and D genes, respectively. The footprinting studies described here, in combination with previous studies on transcription, provide definitive evidence on the position of the B and D gene promoters in S13 and the closely related phage phi X174.
利用DNA酶I足迹法、核酸外切酶III分析和DNA测序相结合的方法，对噬菌体S13基因组中包含B、K和C基因以及大部分A、D和E基因（图谱位置627至2198 ）的区域进行了大肠杆菌RNA聚合酶结合位点分析。分别在936至995位和1779至1848位定位到了两个与B和D基因启动子相对应的高亲和力结合位点。936至995位在基因A中，位于B基因之前；1779至1848位在基因C中，恰好在D基因之前。此外，还分别在C和D基因之前的1527至1538位和1705至1756位鉴定到了两个低亲和力结合位点。本文所述的足迹研究，结合先前关于转录的研究，为B和D基因启动子在S13及密切相关的噬菌体φX174中的位置提供了确凿证据。
