Zhang Xiaoqian, Cao Henghua, Bai Shuyun, Huo Weibang, Ma Yue
University of Chinese Academy of Sciences, Beijing 100049, China; National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China.
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China.
Stem Cell Res. 2017 Apr;20:21-29. doi: 10.1016/j.scr.2017.02.002. Epub 2017 Feb 11.
The combination of non-human primate animals and their induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) provides not only transplantation models for cell-based therapy of heart diseases, but also opportunities for heart-related drug research on both cellular and animal levels. However, the subtypes and electrophysiology properties of non-human primate iPSC-CMs hadn't been detailed characterized. In this study, we generated rhesus monkey induced pluripotent stem cells (riPSCs), and efficiently differentiated them into ventricular or atrial cardiomyocytes by modulating retinoic acid (RA) pathways. Our results revealed that the electrophysiological characteristics and response to canonical drugs of riPSC-CMs were similar with those of human pluripotent stem cell derived CMs. Therefore, rhesus monkeys and their iPSC-CMs provide a powerful and practicable system for heart related biomedical research.
非人灵长类动物及其诱导多能干细胞衍生的心肌细胞（iPSC-CMs）的组合不仅为心脏病的细胞治疗提供了移植模型，也为细胞和动物水平上的心脏相关药物研究提供了机会。然而，非人灵长类iPSC-CMs的亚型和电生理特性尚未得到详细表征。在本研究中，我们生成了恒河猴诱导多能干细胞（riPSCs），并通过调节视黄酸（RA）信号通路将它们高效地分化为心室或心房心肌细胞。我们的结果显示，riPSC-CMs的电生理特征和对标准药物的反应与人类多能干细胞衍生的心肌细胞相似。因此，恒河猴及其iPSC-CMs为心脏相关生物医学研究提供了一个强大且可行的系统。
