巴西东北部卡廷加生物群落中对人类致病的巴尔通体物种感染宠物、自由放养的野生哺乳动物及其体外寄生虫：一项血清学和分子研究。
Bartonella species pathogenic for humans infect pets, free-ranging wild mammals and their ectoparasites in the Caatinga biome, Northeastern Brazil: a serological and molecular study.
作者信息
Fontalvo Mariana Campos, Favacho Alexsandra Rodrigues de Mendonça, Araujo Andreina de Carvalho, Santos Naylla Mayana Dos, Oliveira Glauber Meneses Barboza de, Aguiar Daniel Moura, Lemos Elba Regina Sampaio de, Horta Mauricio Claudio
机构信息
Universidade Federal do Vale do São Francisco (Univasf), Laboratório de Doenças Parasitárias, Petrolina, PE, Brazil.
Instituto Oswaldo Cruz (FIOCRUZ), Laboratório de Hantaviroses e Rickettsioses, Rio de Janeiro, RJ, Brazil.
出版信息
Braz J Infect Dis. 2017 May-Jun;21(3):290-296. doi: 10.1016/j.bjid.2017.02.002. Epub 2017 Feb 27.
This study verified the occurrence of Bartonella spp. in dogs, cats, wild mammals and their ectoparasites in Petrolina and Lagoa Grande Counties, Pernambuco, located in a semi-arid region in Northeastern Brazil. Anti-Bartonella spp. antibodies were detected by indirect immunofluorescence assay (IFA) in 24.8% of dogs (27/109) and in 15% of cats (6/40). Bartonella sp. DNA was identified by PCR performed on DNA extracted from blood and ectoparasites using primers targeting Bartonella sp. gltA and ribC genes in 100% (9/9) of Pulex irritans from Cerdocyon thous, 57.4% (35/61) of P. irritans from dogs, 2.3% (1/43) of Ctenocephalides felis felis from dogs, 53.3% (24/45) of C. felis felis from cats, and 10% (1/10) of Polyplax spp. from Thrichomys apereoides. DNA sequencing identified Bartonella clarridgeiae and Bartonella henselae in C. felis felis from cats, Bartonella rochalimae in P. irritans from dog and C. thous, and Bartonella vinsoni berkhofii in P. irritans from dog.
本研究证实了巴西东北部半干旱地区伯南布哥州佩特罗利纳县和拉戈阿格兰德县的犬、猫、野生哺乳动物及其体表寄生虫中巴尔通体属细菌的存在。通过间接免疫荧光试验（IFA）检测到，24.8%的犬（27/109）和15%的猫（6/40）存在抗巴尔通体属细菌抗体。使用针对巴尔通体属细菌gltA和ribC基因的引物，通过聚合酶链反应（PCR）对从血液和体表寄生虫中提取的DNA进行检测，结果在100%（9/9）的来自食蟹狐的致痒蚤、57.4%（35/61）的来自犬的致痒蚤、2.3%（1/43）的来自犬的猫栉首蚤指名亚种、53.3%（24/45）的来自猫的猫栉首蚤指名亚种以及10%（1/10）的来自阿氏梳趾鼠的多板蚤属中鉴定出巴尔通体属细菌DNA。DNA测序在来自猫的猫栉首蚤指名亚种中鉴定出克拉氏巴尔通体和亨氏巴尔通体，在来自犬和食蟹狐的致痒蚤中鉴定出罗氏巴尔通体，在来自犬的致痒蚤中鉴定出文森巴尔通体伯克霍夫亚种。
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