García Elina V, Hamdi Meriem, Barrera Antonio D, Sánchez-Calabuig María J, Gutiérrez-Adán Alfonso, Rizos Dimitrios
Departamento de Reproducción AnimalInstituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain
Instituto Superior de Investigaciones Biológicas (INSIBIO)CONICET-UNT, and Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina.
Reproduction. 2017 May;153(5):631-643. doi: 10.1530/REP-16-0654. Epub 2017 Mar 1.
Signaling components of bone morphogenetic proteins (BMPs) are expressed in an anatomically and temporally regulated fashion in bovine oviduct. However, a local response of this signaling to the presence of the embryo has yet to be elucidated. The aim of the present study was to evaluate if early embryo-oviduct interaction induces changes in the gene expression of BMP signaling components. For this purpose, we used an co-culture system to investigate the local interaction between bovine oviductal epithelial cells (BOEC) from the isthmus region with early embryos during two developmental periods: before (from the 2-cell to 8-cell stage) or during (from the 8-cell to 16-cell stage) the main phase of embryonic genome activation (EGA). Exposure to embryos, irrespective of the period, significantly reduced the relative abundance of , , , and mRNAs in BOEC. In contrast, embryos that interacted with BOEC before EGA showed a significant increase in the relative abundance of mRNA at the 8-cell stage compared to embryos cultured without BOEC. Moreover, embryos at the 16-cell stage that interacted with BOEC during EGA showed a significant increase in , and mRNA. These results demonstrate that embryo-oviduct interaction induces specific changes in the transcriptional levels of BMP signaling, causing a bidirectional response that reduces the expression levels of this signaling in the oviductal cells while increases them in the early embryo. This suggests that BMP signaling pathway could be involved in an early cross talk between the bovine embryo and the oviduct during the first stages of development.
骨形态发生蛋白（BMPs）的信号传导成分在牛输卵管中以解剖学和时间调控的方式表达。然而，这种信号对胚胎存在的局部反应尚未阐明。本研究的目的是评估早期胚胎与输卵管的相互作用是否会诱导BMP信号传导成分基因表达的变化。为此，我们使用共培养系统来研究来自峡部区域的牛输卵管上皮细胞（BOEC）与处于两个发育阶段的早期胚胎之间的局部相互作用：在胚胎基因组激活（EGA）的主要阶段之前（从2细胞到8细胞阶段）或期间（从8细胞到16细胞阶段）。无论处于哪个时期，与胚胎接触都会显著降低BOEC中、、、和mRNA的相对丰度。相比之下，在EGA之前与BOEC相互作用的胚胎在8细胞阶段时，其mRNA的相对丰度与未与BOEC共培养的胚胎相比显著增加。此外，在EGA期间与BOEC相互作用的16细胞阶段胚胎，其、和mRNA显著增加。这些结果表明，胚胎与输卵管的相互作用会诱导BMP信号传导转录水平的特定变化，导致双向反应，即降低输卵管细胞中该信号的表达水平，同时增加早期胚胎中的表达水平。这表明BMP信号通路可能参与了牛胚胎与输卵管在发育早期阶段的早期相互作用。
