Asano Y, Hiroishi Y, Itakura N, Hirose S, Kajita Y, Nagai T, Yazaki T, Takahashi M
Department of Pediatrics, Fujita Gakuen Health University School of Medicine Aichi, Japan.
Pediatrics. 1987 Dec;80(6):933-6.
Commercially available mouse monoclonal antibodies to human IgG subclass (IgG1 to IgG4) were applied to an enzyme-linked immunosorbent assay to measure IgG subclass-specific antibodies to varicella-zoster virus in children naturally infected with varicella-zoster virus and in varicella vaccine recipients. In children naturally infected with varicella-zoster virus, IgG 1 antibody was detected 2 weeks after onset of the disease in all cases, its activity increased at 1 month after onset, and almost equal antibody value was maintained 10 years after infection. This pattern of antibody response was similar to that of total IgG antibody to varicella-zoster virus after natural infection. On the other hand, low antibody activity was found in IgG2 only at 1 month of the disease. The highest antibody level of IgG3 was shown 2 weeks after onset of the disease; then, it gradually decreased, and no antibody activity was detected 10 years later. IgG4 antibody was first detected 1 month after onset and an almost equal level of antibody was shown 10 years after the disease. After inoculation of children with a live varicella vaccine, in contrast, IgG subclass antibody responses to vaccine recipients were almost equal to those after natural infection.
将市售的抗人IgG亚类（IgG1至IgG4）小鼠单克隆抗体应用于酶联免疫吸附测定，以检测自然感染水痘带状疱疹病毒的儿童和水痘疫苗接种者中针对水痘带状疱疹病毒的IgG亚类特异性抗体。在自然感染水痘带状疱疹病毒的儿童中，所有病例在疾病发作后2周均检测到IgG1抗体，其活性在发病后1个月升高，感染后10年维持几乎相同的抗体值。这种抗体反应模式与自然感染后针对水痘带状疱疹病毒的总IgG抗体相似。另一方面，仅在疾病1个月时发现IgG2抗体活性较低。IgG3的最高抗体水平在疾病发作后2周出现；然后逐渐下降，10年后未检测到抗体活性。IgG4抗体在发病后1个月首次检测到，疾病后10年显示出几乎相同水平的抗体。相比之下，儿童接种水痘活疫苗后，疫苗接种者的IgG亚类抗体反应与自然感染后几乎相同。
