Burnett S, Moreno-Lopez J, Pettersson U
Department of Medical Genetics, University of Uppsala, Sweden.
Nucleic Acids Res. 1987 Nov 11;15(21):8607-20. doi: 10.1093/nar/15.21.8607.
Bovine papillomavirus type 1 DNA replicated to a high copy number in virus-infected bovine fibroblasts. Infected bovine cells were therefore used as a source of RNA for Northern blotting analysis to search for viral transcripts hybridizing to the E1 gene region, implicated in viral DNA replication. Cytoplasmic polyadenylated RNA preparations contained at least five different E1-region transcripts, ranging from 1200 to approximately 4500 nucleotides in length. All of these species contained sequence information from the 5'-end of the E1 open reading frame, but only the largest species included sequences from its central portion. The latter transcript is a candidate mRNA for a stimulatory replication factor (R) previously mapped by genetic experiments (1).
1型牛乳头瘤病毒DNA在病毒感染的牛成纤维细胞中复制至高拷贝数。因此，感染病毒的牛细胞被用作RNA来源，用于Northern印迹分析，以寻找与E1基因区域杂交的病毒转录本，该区域与病毒DNA复制有关。细胞质多聚腺苷酸化RNA制剂包含至少五种不同的E1区域转录本，长度从1200到约4500个核苷酸不等。所有这些转录本都包含来自E1开放阅读框5'端的序列信息，但只有最大的转录本包含来自其中心部分的序列。后一种转录本是先前通过遗传实验定位的刺激复制因子（R）的候选mRNA（1）。
