Chen E Y, Howley P M, Levinson A D, Seeburg P H
Nature. 1982 Oct 7;299(5883):529-34. doi: 10.1038/299529a0.
The complete nucleotide sequence of the doubt-stranded circular DNA of bovine papillomavirus type 1 (BPV-1) was determined. Analysis of this sequence in conjunction with known transcriptional data for the virus provides a basis of determining the organization of the papillomavirus genome. All the major open reading frames are located on the same DNA strand. The region transcribed in BPV-transformed cells contains open frames in all three translational frames whereas the region transcribed in productively infected bovine fibropapillomas is characterized by two large open reading frames partitioned by a single translational stop codon. The localization of sequences diagnostic of promoters and polyadenylation sites suggests that splicing is involved in the biosynthesis of the viral mRNAs. A sequence comparison indicates the genome organization of the bovine papillomavirus and that of the members of the simian virus 40-polyomavirus subgroup to be distinct, suggesting that these two groups of viruses are evolutionarily unrelated.
已确定牛乳头瘤病毒1型（BPV-1）双链环状DNA的完整核苷酸序列。结合该病毒已知的转录数据对这一序列进行分析，为确定乳头瘤病毒基因组的组织形式提供了依据。所有主要的开放阅读框都位于同一条DNA链上。在BPV转化细胞中转录的区域在所有三个翻译框架中都含有开放阅读框，而在产生性感染的牛纤维乳头瘤中转录的区域则以两个大的开放阅读框为特征，这两个开放阅读框由一个单一的翻译终止密码子隔开。诊断启动子和聚腺苷酸化位点的序列定位表明，剪接参与了病毒mRNA的生物合成。序列比较表明，牛乳头瘤病毒的基因组组织形式与猿猴病毒40-多瘤病毒亚组成员的基因组组织形式不同，这表明这两组病毒在进化上没有亲缘关系。
