年轻成年人中RANKL/RANK/OPG多态性与足跟定量超声

RANKL/RANK/OPG Polymorphisms and Heel Quantitative Ultrasound in Young Adults.

作者信息

Correa-Rodríguez María, Schmidt-RioValle Jacqueline, Rueda-Medina Blanca

机构信息

María Correa-Rodríguez, RN, is doctoral student; Jacqueline Schmidt Rio-Valle, PhD, is Professor; and Blanca Rueda-Medina, PhD, is Professor, Department of Nursing, Faculty of Health Sciences, University of Granada, Spain.

出版信息

Nurs Res. 2017 Mar/Apr;66(2):145-151. doi: 10.1097/NNR.0000000000000202.

DOI:10.1097/NNR.0000000000000202

PMID:28252575

Abstract

BACKGROUND

The receptor activator of the nuclear factor-kappa B ligand (RANKL), the receptor activator of nuclear factor-kappa B (RANK), and the osteoprotegerin (OPG) signaling pathway play an important role in the regulation of bone remodeling and osteoclast differentiation. Quantitative ultrasound (QUS) is a relatively recent and noninvasive method providing structural information on microstructure, bone elasticity, and connectivity. However, in contrast to bone mineral density measurements, the possible association of the RANKL/RANK/OPG pathway with heel QUS has not been analyzed.

OBJECTIVES

The aim of this study was to assess, for the first time, the contribution of the RANKL/RANK/OPG pathway genes in the genetic background of heel QUS parameters.

METHODS

Ten single-nucleotide polymorphisms (SNPs) of RANKL (rs9594759, rs12585014, rs7988338, rs2148073), RANK (rs1805034, rs12458117, rs3018362), and OPG (rs4355801, rs3102735, rs2073618) were selected as genetic markers and genotyped using Open Array technology in 575 self-reported Caucasian individuals aged 18-25. Bone mass in the right calcaneus was estimated with QUS to obtain the broadband ultrasound attenuation (BUA) measurement (dB/MHz). Linear regression analyses were performed to test the possible association between the SNPs and BUA.

RESULTS

Linear regression analysis of all the tested SNPs revealed no significant association with the BUA parameter after adjusting for age, gender, weight, height, physical activity, and calcium intake. The lowest p-value was observed for the rs9594759 RANKL polymorphism and heel QUS (p = .06; b* = -.075, 95% CI [-0.960, 0.028]).

CONCLUSION

Our results suggest that the polymorphism of the RANKL, RANK, and OPG genes does not make a significant genetic contribution to heel ultrasound measurements in a population of young Caucasian adults. Further studies replicating the results in independent populations are needed to support these initial findings.

摘要

背景

核因子κB受体活化因子配体（RANKL）、核因子κB受体活化因子（RANK）和骨保护素（OPG）信号通路在骨重塑和破骨细胞分化的调节中起重要作用。定量超声（QUS）是一种相对较新的非侵入性方法，可提供有关微观结构、骨弹性和连通性的结构信息。然而，与骨密度测量不同，RANKL/RANK/OPG通路与足跟QUS之间可能的关联尚未得到分析。

目的

本研究的目的是首次评估RANKL/RANK/OPG通路基因在足跟QUS参数遗传背景中的作用。

方法

选择RANKL（rs9594759、rs12585014、rs7988338、rs2148073）、RANK（rs1805034、rs12458117、rs3018362）和OPG（rs4355801、rs3102735、rs2073618）的10个单核苷酸多态性（SNP）作为遗传标记，并使用开放式阵列技术对575名年龄在18 - 25岁的自我报告为白种人的个体进行基因分型。用QUS估计右跟骨的骨量，以获得宽带超声衰减（BUA）测量值（dB/MHz）。进行线性回归分析以测试SNP与BUA之间可能的关联。