Araya-Cloutier Carla, den Besten Heidy M W, Aisyah Siti, Gruppen Harry, Vincken Jean-Paul
Laboratory of Food Chemistry, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
Laboratory of Food Microbiology, Wageningen University, Wageningen, The Netherlands.
Food Chem. 2017 Jul 1;226:193-201. doi: 10.1016/j.foodchem.2017.01.026. Epub 2017 Jan 7.
The legume plant family (Fabaceae) is a potential source of antimicrobial phytochemicals. Molecular diversity in phytochemicals of legume extracts was enhanced by germination and fungal elicitation of seven legume species, as established by RP-UHPLC-UV-MS. The relationship between phytochemical composition, including different types of skeletons and substitutions, and antibacterial properties of extracts was investigated. Extracts rich in prenylated isoflavonoids and stilbenoids showed potent antibacterial activity against Listeria monocytogenes and methicillin-resistant Staphylococcus aureus at concentrations between 0.05 and 0.1% (w/v). Prenylated phenolic compounds were significantly (p<0.01) correlated with the antibacterial properties of the extracts. Furthermore, the position of the prenyl group within the phenolic skeleton also influenced the antibacterial activity. Overall, prenylated phenolics from legume seedlings can serve multiple purposes, e.g. as phytoestrogens they can provide health benefits and as natural antimicrobials they offer preservation of foods.
豆科植物家族（豆科）是抗菌植物化学物质的潜在来源。通过RP-UHPLC-UV-MS证实，七种豆科植物种子的萌发和真菌诱导提高了豆科植物提取物中植物化学物质的分子多样性。研究了植物化学成分（包括不同类型的骨架和取代基）与提取物抗菌性能之间的关系。富含异戊烯基化异黄酮和芪类化合物的提取物在浓度为0.05%至0.1%（w/v）时，对单核细胞增生李斯特菌和耐甲氧西林金黄色葡萄球菌显示出强大的抗菌活性。异戊烯基化酚类化合物与提取物的抗菌性能显著相关（p<0.01）。此外，异戊烯基在酚类骨架中的位置也影响抗菌活性。总体而言，豆科植物幼苗中的异戊烯基化酚类化合物具有多种用途，例如作为植物雌激素，它们可以提供健康益处，作为天然抗菌剂，它们可以用于食品保鲜。
