Scarpa A, Bonetti F, Menestrina F, Menegazzi M, Chilosi M, Lestani M, Bovolenta C, Zamboni G, Fiore-Donati L
Istituto di Anatomia, University of Verona, Policlinico Borgo Roma, Italy.
Virchows Arch A Pathol Anat Histopathol. 1987;412(1):17-21. doi: 10.1007/BF00750725.
The Southern blot hybridization technique has been applied to study the configuration of immunoglobulin and T-cell receptor genes in 6 cases of the so called mediastinal large cell lymphoma with sclerosis. This lymphoma has been recently recognized as a separate entity among non-Hodgkin lymphomas mainly affecting young adult patients. The B-cell origin of this neoplasm was suggested by means of immunohistochemical analysis. However, the immunophenotypical B-cell related markers used do not always exhibit lineage fidelity. The Southern blot analysis demonstrated the presence of unique heavy and k-light chain immunoglobulin gene rearrangements, establishing genotypically their B-cell origin.
