Rovigatti U, Mirro J, Kitchingman G, Dahl G, Ochs J, Murphy S, Stass S
Blood. 1984 May;63(5):1023-7.
Samples of leukemic cell DNA from 14 children with acute nonlymphocytic leukemia (ANLL) and 4 human myeloid leukemia cell lines were analyzed for rearrangement in the heavy chain region of the immunoglobulin gene. The diagnosis of ANLL was confirmed in all patients by morphological, cytochemical, and immunologic studies. By restriction endonuclease digestion and hybridization with cloned heavy chain immunoglobulin gene probes for the constant (Cmu) and joining (JH) regions, the DNA of 2 patients and 1 cell line (ML-1) was found to contain rearrangements. The DNA from the remaining 12 patients and 3 cell lines was not rearranged (germline configuration). Both patients with apparent immunoglobulin gene rearrangement achieved complete remission on therapy for ANLL. Immunoglobulin gene rearrangement in phenotypically defined ANLL suggests (1) that such changes may not be limited to lymphoid leukemia of B cell lineage, or (2) that, in some patients, the leukemic transforming event may involve stem cells capable of both B cell and myeloid differentiation.
对14例急性非淋巴细胞白血病（ANLL）患儿的白血病细胞DNA样本以及4个人类髓系白血病细胞系进行了免疫球蛋白基因重链区域重排分析。所有患者均通过形态学、细胞化学和免疫学研究确诊为ANLL。通过限制性内切酶消化以及与恒定区（Cmu）和连接区（JH）的克隆重链免疫球蛋白基因探针杂交，发现2例患者和1个细胞系（ML-1）的DNA存在重排。其余12例患者和3个细胞系的DNA未发生重排（种系构型）。两名出现明显免疫球蛋白基因重排的患者在接受ANLL治疗后均实现完全缓解。表型明确的ANLL中免疫球蛋白基因重排提示：（1）此类变化可能不限于B细胞系的淋巴细胞白血病；或（2）在某些患者中，白血病转化事件可能涉及能够进行B细胞和髓系分化的干细胞。
