Galinski M S, Mink M A, Pons M W
Division of Molecular Virology, James N. Gamble Institute of Medical Research, Cincinnati, OH 45219.
Virus Res. 1987 Sep;8(3):205-15. doi: 10.1016/0168-1702(87)90016-5.
The sequence of the genes encoding the fusion (F) and hemagglutinin-neuraminidase (HN) glycoproteins of the human parainfluenza 3 virus was determined by molecular cloning. The genes were cloned by primer extension using genomic 50 S RNA as the template. A series of four overlapping clones was generated from the 3' end of the fusion gene which extended across the gene end and intergenic boundaries of the F-HN and HN-L genes. The F gene extends 1851 nucleotides (inclusive of the putative transcription initiation and polyadenylation signals) and encodes a protein consisting of 539 amino acids (mol wt 60,067). This protein contains four potential sites for N-linked glycosylation in the F1 subunit polypeptide and none in the F2 subunit polypeptide. The lack of a potential site of glycosylation in F2 makes this protein unique compared to other reported paramyxoviral F proteins. The HN gene extends 1888 nucleotides and encodes a protein consisting of 572 amino acids (mol wt 64,255). This protein contains four potential sites for N-linked glycosylation.
通过分子克隆确定了人副流感3病毒融合（F）糖蛋白和血凝素神经氨酸酶（HN）糖蛋白的编码基因序列。以基因组50S RNA为模板，通过引物延伸克隆这些基因。从融合基因的3'端产生了一系列四个重叠克隆，这些克隆跨越了F-HN和HN-L基因的基因末端和基因间边界。F基因延伸1851个核苷酸（包括推定的转录起始和聚腺苷酸化信号），编码一个由539个氨基酸组成的蛋白质（分子量60,067）。该蛋白质在F1亚基多肽中含有四个潜在的N-连接糖基化位点，而在F2亚基多肽中没有。与其他报道的副粘病毒F蛋白相比，F2中缺乏潜在的糖基化位点使得该蛋白具有独特性。HN基因延伸1888个核苷酸，编码一个由572个氨基酸组成的蛋白质（分子量64,255）。该蛋白质含有四个潜在的N-连接糖基化位点。
