Davenport R D, McKeever P E
University of Michigan Medical School, Department of Pathology, Ann Arbor 48109-0602.
Acta Neuropathol. 1987;74(4):362-5. doi: 10.1007/BF00687213.
Immunohistochemical studies of astrocytoma tissue have predominantly shown fibronectin (FN) positivity restricted to vessels and glial fibrillary acidic protein (GFAP) positivity in the parenchyma. Cultured glioma cell lines, however, express both FN and GFAP. We measured the DNA content of explants of gliomas to determine if the ploidy of the FN-positive and GFAP-positive cells differed. Thirty-three explants from four high grade gliomas were cultured on slides. FN and GFAP markers were determined by double immunofluorescence. The slides were stained by the Feulgen method, the explants relocated and the DNA content measured by microdensitometry using the CAS-100 instrument. Human leukocytes applied to the slides were used as a diploid standard. Eleven GFAP-positive explants were hyperdiploid and one hypodiploid. Five FN-positive explants were diploid, three hypodiploid and ten hyperdiploid. One FN-positive explant was biclonal with aneuploid subpopulations. Two hyperdiploid explants, each of which had monoclonal histogram patterns, expressed both FN and GFAP. We conclude that most FN-positive cells, in addition to GFAP-positive cells, from cultured gliomas represent neoplastic cells. These may be present in the tumor in low numbers or may result from marker switching in culture.
对星形细胞瘤组织的免疫组织化学研究主要显示，纤连蛋白（FN）阳性仅限于血管，而胶质纤维酸性蛋白（GFAP）在实质中呈阳性。然而，培养的胶质瘤细胞系同时表达FN和GFAP。我们测量了胶质瘤外植体的DNA含量，以确定FN阳性和GFAP阳性细胞的倍性是否不同。将来自4例高级别胶质瘤的33个外植体培养在载玻片上。通过双重免疫荧光法确定FN和GFAP标记物。用福尔根法对载玻片进行染色，重新定位外植体，并用CAS-100仪器通过显微密度测定法测量DNA含量。应用于载玻片的人白细胞用作二倍体标准。11个GFAP阳性外植体为超二倍体，1个为亚二倍体。5个FN阳性外植体为二倍体，3个为亚二倍体，10个为超二倍体。1个FN阳性外植体为双克隆，具有非整倍体亚群。2个超二倍体外植体，每个都有单克隆直方图模式，同时表达FN和GFAP。我们得出结论，培养的胶质瘤中大多数FN阳性细胞以及GFAP阳性细胞都代表肿瘤细胞。这些细胞可能在肿瘤中数量较少，或者可能是培养过程中标记物转换的结果。
