Chronwall B M, McKeever P E, Kornblith P L
Acta Neuropathol. 1983;59(4):283-7. doi: 10.1007/BF00691494.
Antisera against fibronectin (FN) and glial fibrillary acidic protein (GFAP) were used to combined on frozen sections of surgical biopsies to distinguish between astrocytoma and hemangioblastoma, meningioma, or sarcoma. The nonglial neoplasms contained FN in their parenchyma, whereas the gliomas contained GFAP. The immunofluorescent staining procedure takes 10 min. The immunoperoxidase staining for the permanent files takes 30 min. The stains have been used in this institute for more than 1 year, and 26 tumors have been evaluated.
使用抗纤连蛋白（FN）和胶质纤维酸性蛋白（GFAP）的抗血清与手术活检的冰冻切片相结合，以区分星形细胞瘤与血管母细胞瘤、脑膜瘤或肉瘤。非神经胶质肿瘤的实质中含有FN，而胶质瘤含有GFAP。免疫荧光染色程序需要10分钟。永久性切片的免疫过氧化物酶染色需要30分钟。这些染色方法在该研究所已使用超过1年，并且已对26个肿瘤进行了评估。
