Kochi N, Tani E, Morimura T, Itagaki T
Acta Neuropathol. 1983;59(2):119-26. doi: 10.1007/BF00691597.
The presence of fibronectin (FN) and glial fibrillary acidic protein (GFAP) in two astrocytomas, 17 glioblastomas, and five meningiomas was studied by indirect immunoperoxidase staining of formalin-fixed and paraffin-embedded surgical specimens. Angiogenesis in tumor was scored by the microscopic angiogenesis grading system (MAGS), and plasma FN levels were measured by single radial immunodiffusion. In astrocytomas and glioblastomas, GFAP-positive tumor cells had no FN expression and FN was confined to proliferating vessel walls and the leptomeninges, showing a mutually exclusive FN and GFAP expression. GFAP-positive tumor cells were occasionally surrounded by a network of FN-positive matrix produced by cells derived from the leptomeninges or blood vessels. In meningiomas, FN expression was found in vessel walls and meningioma cells including whorl formations and psammoma bodies. In general, deep immunoperoxidase staining for FN was shown in the endothelial cells and the psammoma bodies. Plasma FN levels were correlated significantly not to the degree of leptomeningeal proliferation but to the MAGS scores in gliomas.
通过对福尔马林固定、石蜡包埋的手术标本进行间接免疫过氧化物酶染色，研究了2例星形细胞瘤、17例胶质母细胞瘤和5例脑膜瘤中纤连蛋白（FN）和胶质纤维酸性蛋白（GFAP）的表达情况。采用微血管生成分级系统（MAGS）对肿瘤血管生成进行评分，通过单向放射免疫扩散法测定血浆FN水平。在星形细胞瘤和胶质母细胞瘤中，GFAP阳性肿瘤细胞无FN表达，FN局限于增殖的血管壁和软脑膜，显示FN和GFAP表达相互排斥。GFAP阳性肿瘤细胞偶尔被源自软脑膜或血管的细胞产生的FN阳性基质网络所包围。在脑膜瘤中，FN表达见于血管壁和脑膜瘤细胞，包括漩涡状结构和砂粒体。一般来说，FN的免疫过氧化物酶染色在内皮细胞和砂粒体中呈强阳性。血浆FN水平与软脑膜增殖程度无显著相关性，但与胶质瘤的MAGS评分显著相关。
