Qian Wei, Wang Yong, Li Rui-Fu, Zhou Xin, Liu Jing, Peng Dai-Zhi
Institute of Burn Research, Southwest Hospital and Tissue Engineering Research Unit, State Key Laboratory of Trauma, Burns, and Combined Injury, 3rd Military Medical University, Chongqing, China (mainland).
Med Sci Monit. 2017 Mar 3;23:1116-1122. doi: 10.12659/msm.903094.
BACKGROUND Lentiviral vectors have been successfully used for human skin cell gene transfer studies. Defining the selection of integration sites for retroviral vectors in the host genome is crucial in risk assessment analysis of gene therapy. However, genome-wide analyses of lentiviral integration sites in human keratinocytes, especially after prolonged growth, are poorly understood. MATERIAL AND METHODS In this study, 874 unique lentiviral vector integration sites in human HaCaT keratinocytes after long-term culture were identified and analyzed with the online tool GTSG-QuickMap and SPSS software. RESULTS The data indicated that lentiviral vectors showed integration site preferences for genes and gene-rich regions. CONCLUSIONS This study will likely assist in determining the relative risks of the lentiviral vector system and in the design of a safe lentiviral vector system in the gene therapy of skin diseases.
背景 慢病毒载体已成功用于人类皮肤细胞基因转移研究。确定逆转录病毒载体在宿主基因组中的整合位点选择对于基因治疗的风险评估分析至关重要。然而，对于人类角质形成细胞中慢病毒整合位点的全基因组分析，尤其是在长期生长后，了解甚少。材料与方法 在本研究中，利用在线工具GTSG-QuickMap和SPSS软件对长期培养后的人类HaCaT角质形成细胞中874个独特的慢病毒载体整合位点进行了鉴定和分析。结果 数据表明慢病毒载体对基因和基因丰富区域表现出整合位点偏好。结论 本研究可能有助于确定慢病毒载体系统的相对风险，并有助于设计用于皮肤病基因治疗的安全慢病毒载体系统。
