• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

McMYB12 转录因子共同调控苹果属海棠花中原花色素和花色苷的生物合成。

McMYB12 Transcription Factors Co-regulate Proanthocyanidin and Anthocyanin Biosynthesis in Malus Crabapple.

机构信息

Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.

Key Laboratory of New Technology in Agricultural Application of Beijing, Beijing University of Agriculture, Beijing, China.

出版信息

Sci Rep. 2017 Mar 3;7:43715. doi: 10.1038/srep43715.

DOI:10.1038/srep43715
PMID:28255171
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5334656/
Abstract

The flavonoid compounds, proanthocyanidins (PAs), protect plants from biotic stresses, contribute to the taste of many fruits, and are beneficial to human health in the form of dietary antioxidants. In this study, we functionally characterized two Malus crabapple R2R3-MYB transcription factors, McMYB12a and McMYB12b, which co-regulate PAs and anthocyanin biosynthesis. McMYB12a was shown to be mainly responsible for upregulating the expression of anthocyanin biosynthetic genes by binding to their promoters, but to be only partially responsible for regulating PAs biosynthetic genes. In contrast, McMYB12b showed preferential binding to the promoters of PAs biosynthetic genes. Overexpression of McMYB12a and McMYB12b in tobacco (Nicotiana tabacum) altered the expression of flavonoid biosynthetic genes and promoted the accumulation of PAs and anthocyanins in tobacco petals. Conversely, transient silencing their expression in crabapple plants, using a conserved gene region, resulted in reduced PAs and anthocyanin production a green leaf phenotype. Meanwhile, transient overexpression of the two genes and silenced McMYB12s in apple (Malus domestica) fruit had a similar effect as overexpression in tobacco and silenced in crabapple. This study reveals a new mechanism for the coordinated regulation of PAs and anthocyanin accumulation in crabapple leaves, which depends on an auto-regulatory balance involving McMYB12a and McMYB12b expression.

摘要

类黄酮化合物，原花青素（PAs），保护植物免受生物胁迫，有助于许多水果的口感，并以膳食抗氧化剂的形式有益于人类健康。在这项研究中，我们功能表征了两个苹果属海棠 R2R3-MYB 转录因子 McMYB12a 和 McMYB12b，它们共同调节 PAs 和花青素生物合成。McMYB12a 主要通过与启动子结合来上调花青素生物合成基因的表达，但仅部分负责调节 PAs 生物合成基因。相比之下，McMYB12b 显示出对 PAs 生物合成基因启动子的优先结合。在烟草（Nicotiana tabacum）中过表达 McMYB12a 和 McMYB12b 改变了类黄酮生物合成基因的表达，并促进了烟草花瓣中 PAs 和花青素的积累。相反，使用保守基因区域瞬时沉默它们在海棠植物中的表达导致 PAs 和花青素产生减少和绿叶表型。同时，在苹果（Malus domestica）果实中转基因过表达这两个基因并沉默 McMYB12s 与在烟草中转基因过表达和在海棠中沉默具有相似的效果。这项研究揭示了一个新的机制，用于协调调控海棠叶片中 PAs 和花青素的积累，这依赖于涉及 McMYB12a 和 McMYB12b 表达的自动调节平衡。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/47deb6f6d81d/srep43715-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/cada41cd2083/srep43715-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/2dc4c2abb6e9/srep43715-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/1cfc75baa746/srep43715-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/e5aac38635fa/srep43715-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/ca84f327a988/srep43715-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/d886da5d9831/srep43715-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/c326f37fd718/srep43715-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/8184408c0b94/srep43715-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/47deb6f6d81d/srep43715-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/cada41cd2083/srep43715-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/2dc4c2abb6e9/srep43715-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/1cfc75baa746/srep43715-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/e5aac38635fa/srep43715-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/ca84f327a988/srep43715-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/d886da5d9831/srep43715-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/c326f37fd718/srep43715-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/8184408c0b94/srep43715-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0156/5334656/47deb6f6d81d/srep43715-f9.jpg

相似文献

1
McMYB12 Transcription Factors Co-regulate Proanthocyanidin and Anthocyanin Biosynthesis in Malus Crabapple.McMYB12 转录因子共同调控苹果属海棠花中原花色素和花色苷的生物合成。
Sci Rep. 2017 Mar 3;7:43715. doi: 10.1038/srep43715.
2
Identification of leucoanthocyanidin reductase and anthocyanidin reductase genes involved in proanthocyanidin biosynthesis in Malus crabapple plants.鉴定苹果属植物原花青素生物合成中参与类黄酮还原酶和花青素还原酶基因。
Plant Physiol Biochem. 2019 Jun;139:141-151. doi: 10.1016/j.plaphy.2019.03.003. Epub 2019 Mar 8.
3
Transcription factor McWRKY71 induced by ozone stress regulates anthocyanin and proanthocyanidin biosynthesis in Malus crabapple.臭氧胁迫诱导的转录因子 McWRKY71 调控苹果属山荆子花色素和原花色素生物合成。
Ecotoxicol Environ Saf. 2022 Mar 1;232:113274. doi: 10.1016/j.ecoenv.2022.113274. Epub 2022 Feb 3.
4
An apple MYB transcription factor, MdMYB3, is involved in regulation of anthocyanin biosynthesis and flower development.一个苹果 MYB 转录因子 MdMYB3 参与调控花色苷生物合成和花发育。
BMC Plant Biol. 2013 Nov 7;13:176. doi: 10.1186/1471-2229-13-176.
5
Application of melatonin promotes anthocyanin accumulation in crabapple leaves.褪黑素的应用促进了海棠叶片花色素苷的积累。
Plant Physiol Biochem. 2019 Sep;142:332-341. doi: 10.1016/j.plaphy.2019.07.024. Epub 2019 Jul 30.
6
A R2R3-MYB transcription factor from Epimedium sagittatum regulates the flavonoid biosynthetic pathway.从淫羊藿中分离得到的一个 R2R3-MYB 转录因子调控黄酮类生物合成途径。
PLoS One. 2013 Aug 1;8(8):e70778. doi: 10.1371/journal.pone.0070778. Print 2013.
7
The Balance of Expression of Dihydroflavonol 4-reductase and Flavonol Synthase Regulates Flavonoid Biosynthesis and Red Foliage Coloration in Crabapples.二氢黄酮醇4-还原酶和黄酮醇合酶表达的平衡调节海棠属植物中黄酮类生物合成和红叶着色
Sci Rep. 2015 Jul 20;5:12228. doi: 10.1038/srep12228.
8
MdMYB9 and MdMYB11 are involved in the regulation of the JA-induced biosynthesis of anthocyanin and proanthocyanidin in apples.MdMYB9和MdMYB11参与了苹果中茉莉酸诱导的花青素和原花青素生物合成的调控。
Plant Cell Physiol. 2015 Apr;56(4):650-62. doi: 10.1093/pcp/pcu205. Epub 2014 Dec 19.
9
The Use of RNA Sequencing and Correlation Network Analysis to Study Potential Regulators of Crabapple Leaf Color Transformation.利用 RNA 测序和关联网络分析研究苹果叶片颜色转变的潜在调控因子。
Plant Cell Physiol. 2018 May 1;59(5):1027-1042. doi: 10.1093/pcp/pcy044.
10
The ethylene response factor MdERF1B regulates anthocyanin and proanthocyanidin biosynthesis in apple.乙烯应答因子 MdERF1B 调控苹果中的花色素苷和原花色素生物合成。
Plant Mol Biol. 2018 Oct;98(3):205-218. doi: 10.1007/s11103-018-0770-5. Epub 2018 Sep 4.

引用本文的文献

1
How the tulip breaking virus creates striped tulips.郁金香碎色病毒如何造就条纹郁金香。
Commun Biol. 2025 Jan 27;8(1):129. doi: 10.1038/s42003-025-07507-z.
2
Loss-of-function mutation in anthocyanidin reductase activates the anthocyanin synthesis pathway in strawberry.花青素还原酶功能丧失突变激活草莓中的花青素合成途径。
Mol Hortic. 2024 Sep 14;4(1):33. doi: 10.1186/s43897-024-00106-2.
3
Chromosomal level genome assemblies of two Malus crabapple cultivars Flame and Royalty.两个苹果品种‘火焰’和‘皇家’的染色体水平基因组组装。

本文引用的文献

1
Transcriptional regulation of secondary metabolism.次级代谢的转录调控。
Funct Plant Biol. 2003 Oct;30(9):913-925. doi: 10.1071/FP03062.
2
The Balance of Expression of Dihydroflavonol 4-reductase and Flavonol Synthase Regulates Flavonoid Biosynthesis and Red Foliage Coloration in Crabapples.二氢黄酮醇4-还原酶和黄酮醇合酶表达的平衡调节海棠属植物中黄酮类生物合成和红叶着色
Sci Rep. 2015 Jul 20;5:12228. doi: 10.1038/srep12228.
3
McMYB10 regulates coloration via activating McF3'H and later structural genes in ever-red leaf crabapple.
Sci Data. 2024 Feb 13;11(1):201. doi: 10.1038/s41597-024-03049-x.
4
Impact of Light and Dark Treatment on Phenylpropanoid Pathway Genes, Primary and Secondary Metabolites in Transgenic Hairy Root Cultures by Overexpressing Transcription Factor .光照与黑暗处理对过表达转录因子的转基因毛状根培养物中苯丙烷类途径基因、初级和次级代谢产物的影响
Life (Basel). 2023 Apr 19;13(4):1042. doi: 10.3390/life13041042.
5
Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Flavonoid Accumulation and Its Underlying Mechanism in Fruits of Distinct Cultivars.整合代谢组学和转录组学分析揭示不同品种果实中黄酮类化合物的差异积累及其潜在机制
Foods. 2022 Aug 21;11(16):2527. doi: 10.3390/foods11162527.
6
Heterologous Expression of Three Transcription Factors Differently Regulated Astragalosides Metabolic Biosynthesis in Hairy Roots.三种转录因子的异源表达对毛状根中黄芪甲苷代谢生物合成的调控存在差异。
Plants (Basel). 2022 Jul 21;11(14):1897. doi: 10.3390/plants11141897.
7
Glucose Supply Induces PsMYB2-Mediated Anthocyanin Accumulation in 'Tai Yang' Cut Flower.葡萄糖供应诱导‘太阳’切花中PsMYB2介导的花青素积累。
Front Plant Sci. 2022 Jun 14;13:874526. doi: 10.3389/fpls.2022.874526. eCollection 2022.
8
Tartary Buckwheat R2R3-MYB Gene Negatively Regulates Anthocyanin and Proanthocyanin Biosynthesis.鞑靼荞麦 R2R3-MYB 基因负调控花色苷和原花色素生物合成。
Int J Mol Sci. 2022 Mar 3;23(5):2775. doi: 10.3390/ijms23052775.
9
ROS1 promotes low temperature-induced anthocyanin accumulation in apple by demethylating the promoter of anthocyanin-associated genes.ROS1 通过使花青素相关基因的启动子去甲基化来促进低温诱导苹果中花青素的积累。
Hortic Res. 2022 Feb 11;9. doi: 10.1093/hr/uhac007.
10
Combining QTL Mapping and Transcriptomics to Decipher the Genetic Architecture of Phenolic Compounds Metabolism in the Conifer White Spruce.结合数量性状基因座定位与转录组学以解析针叶树白云杉中酚类化合物代谢的遗传结构
Front Plant Sci. 2021 May 17;12:675108. doi: 10.3389/fpls.2021.675108. eCollection 2021.
McMYB10 通过激活 McF3'H 和之后的结构基因调控红叶海棠的颜色。
Plant Biotechnol J. 2015 Sep;13(7):948-61. doi: 10.1111/pbi.12331. Epub 2015 Jan 30.
4
MdMYB9 and MdMYB11 are involved in the regulation of the JA-induced biosynthesis of anthocyanin and proanthocyanidin in apples.MdMYB9和MdMYB11参与了苹果中茉莉酸诱导的花青素和原花青素生物合成的调控。
Plant Cell Physiol. 2015 Apr;56(4):650-62. doi: 10.1093/pcp/pcu205. Epub 2014 Dec 19.
5
Characterization of an apple TT2-type R2R3 MYB transcription factor functionally similar to the poplar proanthocyanidin regulator PtMYB134.一种与杨树原花青素调节因子PtMYB134功能相似的苹果TT2型R2R3 MYB转录因子的特性分析
Planta. 2014 Sep;240(3):497-511. doi: 10.1007/s00425-014-2098-y. Epub 2014 Jun 13.
6
Functional characterization of a new grapevine MYB transcription factor and regulation of proanthocyanidin biosynthesis in grapes.葡萄新型 MYB 转录因子功能鉴定及对葡萄原花色素生物合成的调控
J Exp Bot. 2014 Aug;65(15):4433-49. doi: 10.1093/jxb/eru213. Epub 2014 May 23.
7
Functional diversification of grapevine MYB5a and MYB5b in the control of flavonoid biosynthesis in a petunia anthocyanin regulatory mutant.葡萄MYB5a和MYB5b在矮牵牛花青素调控突变体黄酮类生物合成控制中的功能多样化
Plant Cell Physiol. 2014 Mar;55(3):517-34. doi: 10.1093/pcp/pct190. Epub 2013 Dec 20.
8
TRV-GFP: a modified Tobacco rattle virus vector for efficient and visualizable analysis of gene function.TRV-GFP：一种改良的烟草脆裂病毒载体，可高效可视化分析基因功能。
J Exp Bot. 2014 Jan;65(1):311-22. doi: 10.1093/jxb/ert381. Epub 2013 Nov 11.
9
An apple MYB transcription factor, MdMYB3, is involved in regulation of anthocyanin biosynthesis and flower development.一个苹果 MYB 转录因子 MdMYB3 参与调控花色苷生物合成和花发育。
BMC Plant Biol. 2013 Nov 7;13:176. doi: 10.1186/1471-2229-13-176.
10
Involvement of rose aquaporin RhPIP1;1 in ethylene-regulated petal expansion through interaction with RhPIP2;1.玫瑰水通道蛋白 RhPIP1;1 通过与 RhPIP2;1 相互作用参与乙烯调控的花瓣扩张。
Plant Mol Biol. 2013 Oct;83(3):219-33. doi: 10.1007/s11103-013-0084-6. Epub 2013 Jun 8.