Zhang Lili, Yan Li, Zhang Chao, Kong Xin, Zheng Yiqing, Dong Li
Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture and College of Landscape Architecture, Beijing Forestry University, Beijing, China.
Ningxia State Farm, Yinchuan, China.
Front Plant Sci. 2022 Jun 14;13:874526. doi: 10.3389/fpls.2022.874526. eCollection 2022.
Tree peony () is a well-known Chinese ornamental plant with showy flower color. However, the color fading problem during vase time seriously blocks its development in the cut flower market. In this study, we found that exogenous glucose supply improved the color quality of 'Tai Yang' cut flowers with increased total soluble sugar and anthocyanin contents of petals. Besides, the promotion effect of glucose was better than the osmotic control of 3--methylglucose (3OMG) treatment and the glucose analog mannose treatment. The structural genes, including , , , , and , were remarkably upregulated under glucose treatment. Meanwhile, the regulatory genes, including , , , , and , also showed a strong response to glucose treatment. Among these five regulatory genes, showed less response to 3OMG treatment but was highly expressed under glucose and mannose treatments, indicating that may have an important role in the glucose signal pathway. Ectopic overexpression of in resulted in a strong pigmentation in petals and stamens of tobacco flowers accompanied with multiple anthocyanin biosynthetic genes upregulated. More importantly, the overexpression of enhanced the ability of glucose-induced anthocyanin accumulation in seedlings since -overexpressing showed higher expression levels of , , , , , and than those of wild type under glucose treatment. In summary, we suggested that glucose supply promoted petal coloration of 'Tai Yang' cut flower through the signal pathway, and was a key component in this process. Our research made a further understanding of the mechanism that glucose-induced anthocyanin biosynthesis of cut flowers during postharvest development, laying a foundation for color retention technology development of cut flowers.
牡丹（）是一种著名的中国观赏植物，花色艳丽。然而，瓶插期间的颜色褪色问题严重阻碍了其在切花市场的发展。在本研究中，我们发现外源葡萄糖供应提高了‘太阳’切花的颜色品质，花瓣中总可溶性糖和花青素含量增加。此外，葡萄糖的促进作用优于3 - 甲基葡萄糖（3OMG）处理的渗透对照和葡萄糖类似物甘露糖处理。包括、、、、和在内的结构基因在葡萄糖处理下显著上调。同时，包括、、、、和在内的调控基因也对葡萄糖处理表现出强烈反应。在这五个调控基因中，对3OMG处理反应较小，但在葡萄糖和甘露糖处理下高表达，表明可能在葡萄糖信号通路中起重要作用。在中的异位过表达导致烟草花的花瓣和雄蕊强烈色素沉着，同时多个花青素生物合成基因上调。更重要的是，的过表达增强了葡萄糖诱导的幼苗中花青素积累的能力，因为在葡萄糖处理下，过表达的比野生型表现出更高的、、、、和的表达水平。总之，我们认为葡萄糖供应通过信号通路促进了‘太阳’切花的花瓣着色，并且是这一过程中的关键成分。我们的研究进一步了解了采后发育过程中葡萄糖诱导牡丹切花花青素生物合成的机制，为切花保色技术的发展奠定了基础。
