酿酒酵母的五功能芳香酶是单功能结构域的嵌合体。
The pentafunctional arom enzyme of Saccharomyces cerevisiae is a mosaic of monofunctional domains.
作者信息
Duncan K, Edwards R M, Coggins J R
机构信息
Department of Biochemistry, University of Glasgow, U.K.
出版信息
Biochem J. 1987 Sep 1;246(2):375-86. doi: 10.1042/bj2460375.
Abstract
The nucleotide sequence of the Saccharomyces cerevisiae ARO1 gene which encodes the arom multifunctional enzyme has been determined. The protein sequence deduced for the pentafunctional arom polypeptide is 1588 amino acids in length and has a calculated Mr of 174555. Functional regions within the polypeptide chain have been identified by comparison with the sequences of the five monofunctional Escherichia coli enzymes whose activities correspond with those of the arom multifunctional enzyme. The observed homologies demonstrate that the arom polypeptide is a mosaic of functional domains and are consistent with the hypothesis that the ARO1 gene evolved by the linking of ancestral E. coli-like genes.
摘要
已确定酿酒酵母中编码芳香族多功能酶的ARO1基因的核苷酸序列。推导的五功能芳香族多肽的蛋白质序列长度为1588个氨基酸，计算所得的分子量为174555。通过与五种单功能大肠杆菌酶的序列进行比较，确定了多肽链内的功能区域，这些酶的活性与芳香族多功能酶的活性相对应。观察到的同源性表明，芳香族多肽是功能域的镶嵌体，这与ARO1基因是由类似大肠杆菌的祖先基因连接进化而来的假设一致。
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