Duncan K, Edwards R M, Coggins J R
Department of Biochemistry, University of Glasgow, Scotland.
FEBS Lett. 1988 Dec 5;241(1-2):83-8. doi: 10.1016/0014-5793(88)81036-6.
The ARO1 gene of Saccharomyces cerevisiae encodes the arom multifunctional enzyme. Specific inhibitors of amino acid biosynthesis have been used to obtain evidence that expression of a cloned ARO1 gene is regulated in response to amino acid limitation. Northern blot analysis and sequence studies indicate that ARO1 is regulated by the well characterised S. cerevisiae 'general control' mechanism. This provides a very economical means of simultaneously tailoring the synthesis of five shikimate pathway enzymes to the needs of the cell.
酿酒酵母的ARO1基因编码芳香族多功能酶。已使用氨基酸生物合成的特异性抑制剂来获取证据，证明克隆的ARO1基因的表达受氨基酸限制的影响而受到调控。Northern印迹分析和序列研究表明，ARO1受酿酒酵母特征明确的“一般控制”机制调控。这提供了一种非常经济的方式，可同时根据细胞需求调整莽草酸途径中五种酶的合成。
