花生四烯酸和15(S)-羟基-5,8,11-顺式-13-反式-二十碳四烯酸通过单核细胞衍生的中性粒细胞活化因子调节人多形核中性粒细胞的活化。

Arachidonic acid and 15(S)-hydroxy-5,8,11-cis-13-trans-eicosatetraenoic acid modulate human polymorphonuclear neutrophil activation by monocyte derived neutrophil activating factor.

作者信息

Smith R J, Sam L M, Justen J M, Bundy G L, Fitzpatrick F A, Wynalda M A

机构信息

Department of Hypersensitivity Diseases Research, Upjohn Company, Kalamazoo, MI 49001.

出版信息

Biochem Biophys Res Commun. 1987 Oct 29;148(2):636-45. doi: 10.1016/0006-291x(87)90924-7.

DOI:10.1016/0006-291x(87)90924-7

PMID:2825667

Abstract

Exposure of human polymorphonuclear neutrophils (PMN) to human monocyte derived neutrophil activating factor(s) (NAF) resulted in a concentration-dependent extracellular release of granule constituents. NAF also induced the generation of 5(S),12(R)-dihydroxy-6,14-cis-8,10-trans-eicosatetraenoic acid [Leukotriene B4 (LTB4)] by PMNs which was enhanced in the presence of exogenous arachidonic acid (AA). In contrast to its enhancing effect on LTB4 production, AA inhibited NAF-stimulated PMN degranulation. 15(S)-hydroxy-5,8,11-cis-13-trans-eicosatetraenoic acid (15-HETE), a product of the 15-lipoxy-genation of AA in PMNS, caused a concentration-dependent suppression of degranulation and LTB4 generation by PMNs in contact with NAF. 15-HETE also inhibited the rise in cytosolic-free calcium [( Ca2+]i) observed in NAF activated PMNs. These data suggest that AA and a 15-lipoxygenase product modulate the NAF-associated activation pathway in human PMNs.

摘要

人多形核中性粒细胞（PMN）暴露于源自人单核细胞的中性粒细胞激活因子（NAF）会导致颗粒成分呈浓度依赖性的细胞外释放。NAF还诱导PMN生成5(S),12(R)-二羟基-6,14-顺式-8,10-反式-二十碳四烯酸[白三烯B4（LTB4）]，在外源性花生四烯酸（AA）存在的情况下这种生成会增强。与它对LTB4产生的增强作用相反，AA抑制NAF刺激的PMN脱颗粒。15(S)-羟基-5,8,11-顺式-13-反式-二十碳四烯酸（15-HETE）是AA在PMN中经15-脂氧合作用产生的产物，它会对与NAF接触的PMN的脱颗粒和LTB4生成产生浓度依赖性的抑制作用。15-HETE还抑制在NAF激活的PMN中观察到的胞质游离钙[（Ca2+）i]升高。这些数据表明，AA和一种15-脂氧合酶产物调节人PMN中与NAF相关的激活途径。