Fanali Salvatore
Institute of Chemical Methodologies, Italian National Research Council, Monterotondo, Italy.
Electrophoresis. 2017 Aug;38(15):1822-1829. doi: 10.1002/elps.201600573. Epub 2017 Mar 24.
Nano-liquid chromatography (nano-LC) and CEC are microfluidic techniques mainly used for analytical purposes. They have been applied to the separation and analysis of a large number of compounds, e.g., peptides, proteins, drugs, enantiomers, antibiotics, pesticides, nutraceutical, etc. Analytes separation is carried out into capillaries containing selected stationary phase. The mobile phase is moved either by a pump (nano-LC) or by an EOF, respectively. The two tools can offer some advantages over conventional techniques, e.g., high selectivity, separation efficiency, resolution, short analysis time and consumption of low volumes of mobile phase. Flow rates in the range 50-800 nL/min are usually applied. The low flow rate reduces the chromatographic dilution increasing the mass sensitivity. Special attention must be paid in avoiding peak dispersion selecting the appropriate detector, injector and tube connection. Finally due to the low flow rate these microfluidic techniques can be easily coupled with mass spectrometry.
纳升液相色谱法（nano-LC）和毛细管电色谱法（CEC）是主要用于分析目的的微流控技术。它们已被应用于大量化合物的分离和分析，例如肽、蛋白质、药物、对映体、抗生素、农药、营养保健品等。分析物的分离在含有选定固定相的毛细管中进行。流动相分别通过泵（纳升液相色谱法）或电渗流移动。这两种工具相对于传统技术具有一些优势，例如高选择性、分离效率、分辨率、短分析时间和低流动相消耗量。通常采用50 - 800纳升/分钟范围内的流速。低流速减少了色谱稀释，提高了质量灵敏度。在选择合适的检测器、进样器和管路连接以避免峰展宽时必须格外注意。最后，由于流速低，这些微流控技术可以很容易地与质谱联用。
