编码20 kDa变异型人生长激素的cDNA分子克隆及可变剪接机制

Molecular cloning of cDNA encoding 20 kDa variant human growth hormone and the alternative splicing mechanism.

作者信息

Masuda N, Watahiki M, Tanaka M, Yamakawa M, Shimizu K, Nagai J, Nakashima K

机构信息

Department of Biochemistry, Mie University School of Medicine, Japan.

出版信息

Biochim Biophys Acta. 1988 Jan 25;949(1):125-31. doi: 10.1016/0167-4781(88)90062-0.

DOI:10.1016/0167-4781(88)90062-0

PMID:2825811

Abstract

cDNA encoding the 20 kDa variant form of human growth hormone has been cloned, and its sequence analysis verified the alternative splicing mechanism for the mRNA synthesis. The cDNA sequence lacked 45 nucleotides corresponding to the sequence of 15 amino acids in the 22 kDa form of growth hormone. The cDNA clones for the 20 kDa variant hormone had the homogeneous 5'-ends, while the clones for the 22 kDa form showed a minor heterogenity, having two transcription initiation sites. The percentage of 20 kDa variant cDNA clones was approx. 7.7% of the total human growth hormone cDNA clones, consistent with the contents of 20 kDa hormone protein in human anterior pituitary and plasma.

摘要

编码人生长激素20 kDa变体形式的cDNA已被克隆，其序列分析证实了mRNA合成的可变剪接机制。该cDNA序列缺少对应于22 kDa生长激素形式中15个氨基酸序列的45个核苷酸。20 kDa变体激素的cDNA克隆具有均一的5'端，而22 kDa形式的克隆显示出轻微的异质性，有两个转录起始位点。20 kDa变体cDNA克隆的比例约为人类生长激素cDNA克隆总数的7.7%，这与人类垂体前叶和血浆中20 kDa激素蛋白的含量一致。