Özek Gulmira, Schepetkin Igor A, Utegenova Gulzhakhan A, Kirpotina Liliya N, Andrei Spencer R, Özek Temel, Başer Kemal Hüsnü Can, Abidkulova Karime T, Kushnarenko Svetlana V, Khlebnikov Andrei I, Damron Derek S, Quinn Mark T
Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir, Turkey.
Department of Microbiology and Immunology, Montana State University, Bozeman, Montana, USA.
J Leukoc Biol. 2017 Jun;101(6):1361-1371. doi: 10.1189/jlb.3A1216-518RR. Epub 2017 Mar 3.
Essential oil extracts from have been used traditionally in Kazakhstan for treatment of inflammation and other illnesses. Because little is known about the biologic activity of these essential oils that contributes to their therapeutic properties, we analyzed their chemical composition and evaluated their phagocyte immunomodulatory activity. The main components of the extracted essential oils were ()-propenyl -butyl disulfide (15.7-39.4%) and ()-propenyl -butyl disulfide (23.4-45.0%). essential oils stimulated [Ca] mobilization in human neutrophils and activated ROS production in human neutrophils and murine bone marrow phagocytes. Activation of human neutrophil [Ca] flux by essential oils was dose-dependently inhibited by capsazepine, a TRPV1 channel antagonist, indicating that TRPV1 channels mediate this response. Furthermore, essential oils stimulated Ca influx in TRPV1 channel-transfected HEK293 cells and desensitized the capsaicin-induced response in these cells. Additional molecular modeling with known TRPV1 channel agonists suggested that the active component is likely to be ()-propenyl -butyl disulfide. Our results provide a cellular and molecular basis to explain at least part of the beneficial therapeutic properties of FEOs.
在哈萨克斯坦，传统上一直使用从[具体来源未提及]中提取的精油来治疗炎症和其他疾病。由于对这些精油有助于其治疗特性的生物活性了解甚少，我们分析了它们的化学成分并评估了它们的吞噬细胞免疫调节活性。提取的精油的主要成分是（）-丙烯基-丁基二硫化物（15.7 - 39.4%）和（）-丙烯基-丁基二硫化物（23.4 - 45.0%）。[具体来源未提及]精油刺激人中性粒细胞中的[Ca]动员，并激活人中性粒细胞和小鼠骨髓吞噬细胞中的活性氧生成。TRPV1通道拮抗剂辣椒素能剂量依赖性地抑制[具体来源未提及]精油对人中性粒细胞[Ca]通量的激活，表明TRPV1通道介导了这种反应。此外，[具体来源未提及]精油刺激转染了TRPV1通道的HEK293细胞中的Ca内流，并使这些细胞中辣椒素诱导的反应脱敏。用已知的TRPV1通道激动剂进行的额外分子建模表明，活性成分可能是（）-丙烯基-丁基二硫化物。我们的结果提供了一个细胞和分子基础，以解释至少部分[具体来源未提及]精油的有益治疗特性。
