Biomedical Research Center, Medical College of Villa Clara, Ave Acueducto y Circunvalación, CP 50200, Santa Clara, Cuba.
Universidad Central Marta Abreu de Las Villas, Carretera a Camajuaní km 5.5, CP 54830, Santa Clara, Cuba.
Biol Trace Elem Res. 2017 Oct;179(2):237-246. doi: 10.1007/s12011-017-0962-x. Epub 2017 Mar 3.
The present study aimed to investigate, in the streptozotocin-induced mild diabetic rat model, the zinc (Zn), copper (Cu), iron (Fe), calcium (Ca), and magnesium (Mg) concentration in serum, liver, and kidney tissues, and urine samples from adult Wistar rats treated neonatally with streptozotocin (STZ). Diabetes was induced by subcutaneous administration of streptozotocin (100 mg/Kg) in female Wistar rats of 2 days old (STZ, n = 10). Control group (CG, n = 10) received only sodium-citrate buffer. The mineral concentrations were measured by atomic absorption spectrophotometry. The validity and accuracy were checked by conventional methods. STZ neonatal injection successfully leaded to mild diabetes in the adult rats. Serum concentrations of Zn, Cu, Fe, Ca, and Mg showed no changes (p > 0.05) due to diabetes. The Zn, Fe, Ca, and Mg concentrations in liver and kidney tissues were not different (p > 0.05) between STZ and CG. The mean values of Cu were higher (p < 0.05) in liver and kidney samples from STZ as compared to CG. Urine minerals concentrations (Zn, Cu, Fe and Ca) in STZ-rats group were lower (p < 0.05) than CG. However, the content of all evaluated minerals in the excreted urine were higher (p < 0.01) in STZ-rats during a 24 h collection period. Urinary excretion of Zn, Cu, Fe, Ca, and Mg was strongly correlated with urinary volume during the 24 h period (r > 0.7; p < 0.001). Observed changes in mineral metabolism of STZ-induced mild diabetes model could be due to the endocrine imbalance associated with the diabetic condition.
本研究旨在研究链脲佐菌素（STZ）诱导的轻度糖尿病大鼠模型中，成年 Wistar 大鼠经新生期 STZ 处理后血清、肝、肾组织和尿液中锌（Zn）、铜（Cu）、铁（Fe）、钙（Ca）和镁（Mg）的浓度。2 天大的雌性 Wistar 大鼠（STZ，n=10）经皮下注射 STZ（100mg/kg）诱导糖尿病。对照组（CG，n=10）仅接受柠檬酸钠缓冲液。通过原子吸收分光光度法测量矿物质浓度。通过常规方法检查有效性和准确性。新生期 STZ 注射成功导致成年大鼠轻度糖尿病。由于糖尿病，血清中 Zn、Cu、Fe、Ca 和 Mg 浓度没有变化（p>0.05）。肝、肾组织中 Zn、Fe、Ca 和 Mg 浓度在 STZ 和 CG 之间无差异（p>0.05）。与 CG 相比，STZ 肝、肾组织中 Cu 的平均值较高（p<0.05）。STZ 大鼠组尿中矿物质浓度（Zn、Cu、Fe 和 Ca）较低（p<0.05）。然而，在 24 小时收集期间，STZ 大鼠排泄的所有评估矿物质的含量均较高（p<0.01）。Zn、Cu、Fe、Ca 和 Mg 的尿排泄量与 24 小时期间的尿量呈强相关（r>0.7；p<0.001）。STZ 诱导的轻度糖尿病模型中矿物质代谢的变化可能是由于与糖尿病相关的内分泌失衡。
