Ptushenko V V, Solovchenko A E
Lomonosov Moscow State University, Belozersky Research Institute of Physico-Chemical Biology, Moscow, 119991, Russia.
Biochemistry (Mosc). 2016 Dec;81(12):1531-1537. doi: 10.1134/S0006297916120142.
The symbiotic unicellular chlorophyte Desmodesmus sp. IPPAS-2014 capable of growth at extremely high CO2 levels prohibitive for most other microalgae is an interesting model for studies of CO2 tolerance mechanisms and a promising organism for CO2 biocapture. We studied the initial (0-60 min) phase of acclimation of this microalga to an abrupt decrease in pH of the medium sparged with air/20% CO2 mixture. Acclimation of the culture to these conditions was accompanied by a sharp decrease in photochemical activity of the chloroplast followed by its recovery with a characteristic time of 10-50 min. We hypothesize that acidification of the cultivation medium by dissolving CO2 plays a key role in the observed decrease in the photochemical activity. The possible role of photosynthetic apparatus tolerance to abrupt acidification in overall high tolerance of symbiotic microalgae to extremely high CO2 levels is discussed.
共生单细胞绿藻Desmodesmus sp. IPPAS - 2014能够在极高二氧化碳水平下生长，而这种水平对大多数其他微藻来说是难以承受的，它是研究二氧化碳耐受机制的有趣模型，也是用于二氧化碳生物捕获的有前景的生物。我们研究了这种微藻对用空气/20%二氧化碳混合物鼓泡的培养基pH值突然下降的初始（0 - 60分钟）适应阶段。培养物对这些条件的适应伴随着叶绿体光化学活性的急剧下降，随后在10 - 50分钟的特征时间内恢复。我们推测，通过溶解二氧化碳使培养基酸化在观察到的光化学活性下降中起关键作用。讨论了光合装置对突然酸化的耐受性在共生微藻对极高二氧化碳水平的整体高耐受性中的可能作用。
