Gorelova Olga, Baulina Olga, Solovchenko Alexei, Selyakh Irina, Chivkunova Olga, Semenova Larisa, Scherbakov Pavel, Burakova Olga, Lobakova Elena
Department of Bioengineering Faculty of Biology, Moscow State University, GSP-1, 119234, Moscow, Russia.
Arch Microbiol. 2015 Mar;197(2):181-95. doi: 10.1007/s00203-014-1036-5. Epub 2014 Sep 20.
A quantitative micromorphometric study of the cell compartment rearrangements was performed in a symbiotic chlorophyte Desmodesmus sp. 3Dp86E-1 grown on nitrogen (N) replete or N-free medium under 480 μmol PAR quanta m(-2) s(-1). The changes in the chloroplast, intraplastidial, and cytoplasmic inclusions induced by high light (HL) and N starvation were similar to those characteristic of free-living chlorophytes. The N-sufficient culture responded to HL by a transient swelling of the thylakoid lumen and a decline in photosynthetic efficiency followed by its recovery. In the N-starving cells, a more rapid expansion and thylakoid swelling occurred along with the irreversible decline in the photosynthetic efficiency. Differential induction of starch grains, oil bodies, and cell wall polysaccharides depending on the stress exposure and type was recorded. Tight relationships between the changes in the assimilatory and storage compartments in the stressed Desmodesmus sp. cells were revealed.
对一种共生绿藻Desmodesmus sp. 3Dp86E-1进行了细胞区室重排的定量微观形态学研究，该绿藻在480 μmol PAR量子m(-2) s(-1)条件下，生长于富含氮（N）或无氮培养基上。高光（HL）和氮饥饿诱导的叶绿体、质体内和细胞质内含物的变化与自由生活绿藻的特征变化相似。氮充足的培养物对高光的反应是类囊体腔短暂肿胀，光合效率下降，随后恢复。在氮饥饿细胞中，类囊体更迅速地扩张和肿胀，同时光合效率不可逆地下降。记录了根据应激暴露和类型对淀粉粒、油体和细胞壁多糖的差异诱导。揭示了应激的Desmodesmus sp.细胞中同化和储存区室变化之间的紧密关系。
