宿主细胞对脑心肌炎病毒（EMCV）反应的转录谱分析。
Transcriptional profiling of host cell responses to encephalomyocarditis virus (EMCV).
作者信息
Wei Jia, Zhang Haixia, Li Xiangrong, Li Qiongyi, Ma Zhongren, Bai Jialin, Qiao Zilin, Feng Ruofei
机构信息
The Key Bio-engineering and Technology Laboratory of SEAC, Northwest Minzu University, No. 1 Xibeixincun, Lanzhou, 730030, People's Republic of China.
School of Life Science and Bioengineering, Northwest Minzu University, No. 1 Xibeixincun, Lanzhou, 730030, People's Republic of China.
出版信息
Virol J. 2017 Mar 4;14(1):45. doi: 10.1186/s12985-017-0718-4.
BACKGROUD
Encephalomyocarditis virus (EMCV) has been discovered on pig farms worldwide and can cause myocarditis in piglets and reproductive failure in sows. However, little is known about the host transcriptional responses to infection and host-pathogen interactions.
METHODS
In this study, transcription profiling was performed by Illumina RNA-Sequencing (RNA-seq) to identify EMCV induced differentially expressed genes in BHK-21 cells at serial time points (12, 24, and 30 h post infection (hpi)), using mock infected cells as control.
RESULTS
We identified 237, 241, and 207 differentially expressed genes (DEGs) respectively, majority of which were up-regulated. A large number of DEGs clustered into host defense, cellular signaling and metabolism categories. Moreover, short time series expression analysis revealed that 12 hpi was an important time point for expression change, indicating host virus resistance.
CONCLUSIONS
This RNA-seq analysis provides the first data for understanding the network of virus host interactions under EMCV infection in vitro, and for identifying host components which involved in the virus infection course.
背景
脑心肌炎病毒（EMCV）已在全球范围内的养猪场中被发现，可导致仔猪心肌炎和母猪繁殖失败。然而，关于宿主对感染的转录反应以及宿主-病原体相互作用知之甚少。
方法
在本研究中，使用未感染的细胞作为对照，通过Illumina RNA测序（RNA-seq）进行转录谱分析，以鉴定在连续时间点（感染后12、24和30小时（hpi））EMCV诱导的BHK-21细胞中差异表达的基因。
结果
我们分别鉴定出237、241和207个差异表达基因（DEG），其中大多数为上调基因。大量DEG聚集在宿主防御、细胞信号传导和代谢类别中。此外，短时间序列表达分析表明，感染后12小时是表达变化的重要时间点，表明宿主对病毒的抵抗力。
结论
该RNA-seq分析为理解体外EMCV感染下病毒-宿主相互作用网络以及鉴定参与病毒感染过程的宿主成分提供了首批数据。
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