Ezaki T, Kanematsu T, Okamura T, Sonoda T, Sugimachi K
Second Department of Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Cancer. 1988 Jan 1;61(1):106-9. doi: 10.1002/1097-0142(19880101)61:1<106::aid-cncr2820610118>3.0.co;2-r.
Cell nuclear DNA content was microspectrophotometrically measured in 60 specimens of clinical hepatocellular carcinoma (HCC). The DNA distribution pattern was classified into two types, diploid and nondiploid, according to the degree of dispersion on the DNA histogram. Among these 60 specimens, 26 had an HCC of less than 5 cm in diameter. In these 26, 17 (65%) had the diploid pattern and 9 (35%) had the nondiploid pattern. In the remaining 34 specimens with a large HCC exceeding 5 cm in diameter, the diploid pattern was seen in only six (18%), and the nondiploid pattern was seen in 28 (82%). Following increase in tumor size, the number of cases of nondiploid increased. The DNA pattern closely correlated with the morphologic grading and age of patients, yet there was no correlation among DNA pattern and presence of hepatitis B surface antigen (HBsAg) or presence of liver cirrhosis. Regarding the prognosis of patients with HCC, there was no statistic difference between DNA pattern and prognosis. The present study suggests that the DNA pattern relates to the biologic characteristics of the growth pattern of HCC. However, the DNA pattern showed no significant correlation with survival rates of patients with HCC who underwent hepatic resection.
采用显微分光光度法对60例临床肝细胞癌（HCC）标本的细胞核DNA含量进行了测定。根据DNA直方图上的离散程度，将DNA分布模式分为二倍体和非二倍体两种类型。在这60例标本中，26例为直径小于5cm的HCC。在这26例中，17例（65%）为二倍体模式，9例（35%）为非二倍体模式。在其余34例直径超过5cm的大HCC标本中，仅6例（18%）为二倍体模式，28例（82%）为非二倍体模式。随着肿瘤大小的增加，非二倍体病例数增加。DNA模式与患者的形态学分级和年龄密切相关，但DNA模式与乙型肝炎表面抗原（HBsAg）的存在或肝硬化的存在之间没有相关性。关于HCC患者的预后，DNA模式与预后之间没有统计学差异。本研究表明，DNA模式与HCC生长模式的生物学特性有关。然而，DNA模式与接受肝切除的HCC患者的生存率没有显著相关性。
