Duan Zhenghua, Xing Yanshuai, Feng Zhitong, Zhang Huiyuan, Li Caixia, Gong Zhiyuan, Wang Lei, Sun Hongwen
School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China.
Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Nankai University, Tianjin 300071, China.
Environ Pollut. 2017 May;224:706-713. doi: 10.1016/j.envpol.2017.02.055. Epub 2017 Mar 1.
As an emerging contaminant, 1-H-benzotriazole (1H-BTR) has been detected in the engineered and natural aquatic environments, which usually coexists with heavy metals and causes combined pollution. In the present study, wild-type and transgenic zebrafish Danio rerio were used to explore the acute toxicity as well as the single and joint hepatotoxicity of cadmium (Cd) and 1H-BTR. Although the acute toxicity of 1H-BTR to zebrafish was low, increased expression of liver-specific fatty acid binding protein was observed in transgenic zebrafish when the embryos were exposed to 5.0 μM of 1H-BTR for 30 days. Besides, co-exposure to 1H-BTR not only reduced the acute toxic effects induced by Cd, but also alleviated the Cd-induced liver atrophy in transgenic fish. Correspondingly, effects of combined exposure to 1H-BTR on the Cd-induced expressions of several signal pathway-related genes and superoxide dismutase and glutathione-s-transferase proteins were studied. Based on the determination of Cd bioaccumulation in fish and the complexing stability constant (β) of Cd-BTR complex in solution, the detoxification mechanism of co-existing 1H-BTR on Cd to the zebrafish was discussed.
作为一种新兴污染物，1-H-苯并三唑（1H-BTR）已在工程和天然水生环境中被检测到，它通常与重金属共存并导致复合污染。在本研究中，使用野生型和转基因斑马鱼（Danio rerio）来探究镉（Cd）和1H-BTR的急性毒性以及单一和联合肝毒性。尽管1H-BTR对斑马鱼的急性毒性较低，但当胚胎暴露于5.0 μM的1H-BTR 30天时，在转基因斑马鱼中观察到肝脏特异性脂肪酸结合蛋白的表达增加。此外，共同暴露于1H-BTR不仅降低了Cd诱导的急性毒性作用，还减轻了转基因鱼中Cd诱导的肝脏萎缩。相应地，研究了共同暴露于1H-BTR对Cd诱导的几种信号通路相关基因以及超氧化物歧化酶和谷胱甘肽-S-转移酶蛋白表达的影响。基于对鱼体内Cd生物积累的测定以及溶液中Cd-BTR络合物的络合稳定常数（β），探讨了共存的1H-BTR对斑马鱼体内Cd的解毒机制。
