State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Nanomedicine. 2017 Jul;13(5):1809-1819. doi: 10.1016/j.nano.2017.02.011. Epub 2017 Mar 1.
Adipose-derived stem cells (ADSCs) are considered to be ideal stem cell sources for bone regeneration owing to their ability to differentiate into osteo-like cells. Therefore, they have attracted increasing attention in recent years. Tetrahedral DNA nanostructures (TDNs), a new type of DNA-based biomaterials, have shown great potential for biomedical applications. In the present work, we aimed to investigate the role played by TDNs in osteogenic differentiation and proliferation of ADSCs and tried to explore if the canonical Wnt signal pathway could be the vital biological mechanism driving these cellular responses. Upon exposure to TDNs, ADSCs proliferation and osteogenic differentiation were significantly enhanced, accompanied by the up-regulation of genes correlated with the Wnt/β-catenin pathway. In conclusion, our results indicate that TDNs are crucial regulators of the increase in osteogenic potential and ADSCs proliferation, and this noteworthy discovery could provide a promising novel approach toward ADSCs-based bone defect regeneration.
脂肪来源干细胞（ADSCs）因其能够分化为成骨样细胞而被认为是骨再生的理想干细胞来源。因此，近年来它们受到了越来越多的关注。四面体 DNA 纳米结构（TDN）作为一种新型的基于 DNA 的生物材料，在生物医学应用中显示出巨大的潜力。在本工作中，我们旨在研究 TDN 在 ADSC 成骨分化和增殖中的作用，并试图探讨经典 Wnt 信号通路是否可能是驱动这些细胞反应的重要生物学机制。暴露于 TDN 后，ADSCs 的增殖和成骨分化明显增强，同时与 Wnt/β-catenin 通路相关的基因表达上调。总之，我们的结果表明 TDN 是增加成骨潜能和 ADSC 增殖的关键调节剂，这一有意义的发现可能为基于 ADSC 的骨缺损再生提供一种有前途的新方法。
