State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology , Sichuan University , Chengdu 610041 , P. R. China.
ACS Appl Mater Interfaces. 2018 Mar 7;10(9):7892-7900. doi: 10.1021/acsami.8b00833. Epub 2018 Feb 20.
Stem cell-based therapy is considered a promising approach for the repair of nervous tissues. Neural stem cells (NSCs) cannot proliferate or differentiate efficiently; hence, different biomaterials have been explored to improve NSC proliferation and differentiation. However, these agents either had low bioavailability or poor biocompatibility. In this work, our group investigated the effects of tetrahedral DNA nanostructures (TDNs), a novel DNA biological material, on the self-renew and differentiation of neuroectodermal (NE-4C) stem cells. We observed that TDN treatment promoted self-renew of the stem cells via activating the Wnt/β -catenin pathway. In addition, our findings suggested that NE-4C stem cells' neuronal differentiation could be promoted effectively by TDNs via inhibiting the notch signaling pathway. In summary, this is the first report about the effects of TDNs on the proliferation and differentiation of NE-4C stem cells and the results demonstrate that TDNs have a great potential in nerve tissue regeneration.
基于干细胞的治疗被认为是修复神经组织的一种有前途的方法。神经干细胞（NSCs）不能有效地增殖或分化；因此，已经探索了不同的生物材料来提高 NSC 的增殖和分化。然而，这些试剂要么生物利用度低，要么生物相容性差。在这项工作中，我们小组研究了四面体 DNA 纳米结构（TDN），一种新型的 DNA 生物材料，对神经外胚层（NE-4C）干细胞自我更新和分化的影响。我们观察到 TDN 通过激活 Wnt/β-catenin 途径促进干细胞的自我更新。此外，我们的研究结果表明，TDN 可以通过抑制 notch 信号通路有效促进 NE-4C 干细胞的神经元分化。总之，这是第一篇关于 TDN 对 NE-4C 干细胞增殖和分化影响的报告，结果表明 TDN 在神经组织再生方面具有巨大的潜力。
