de Carvalho Márcio, Acencio Márcio Luís, Laitz Alessandra Vasconcellos Nunes, de Araújo Laura Migliorini, de Lara Campos Arcuri Mariana, do Nascimento Leandro Costa, Maia Ivan G
Departamento de Genética, Instituto de Biociências, UNESP, Botucatu, SP, 18618-970, Brazil.
Departamento de Física e Biofísica, Instituto de Biociências, UNESP, Botucatu, SP, Brazil.
Plant Cell Rep. 2017 Jun;36(6):887-900. doi: 10.1007/s00299-017-2117-0. Epub 2017 Mar 4.
Overexpression of a tomato TCTP impacts plant biomass production and performance under stress. These phenotypic alterations were associated with the up-regulation of genes mainly related to photosynthesis, fatty acid metabolism and water transport. The translationally controlled tumor protein (TCTP) is a multifaceted and highly conserved eukaryotic protein. In plants, despite the existence of functional data implicating this protein in cell proliferation and growth, the detailed physiological roles of many plant TCTPs remain poorly understood. Here we focused on a yet uncharacterized TCTP from tomato (SlTCTP). We show that, when overexpressed in tobacco, SlTCTP may promote plant biomass production and affect performance under salt and osmotic stress. Transcriptomic analysis of the transgenic plants revealed the up-regulation of genes mainly related to photosynthesis, fatty acid metabolism and water transport. This induced photosynthetic gene expression was paralleled by an increase in the photosynthetic rate and stomatal conductance of the transgenic plants. Moreover, the transcriptional modulation of genes involved in ABA-mediated regulation of stomatal movement was detected. On the other hand, genes playing a pivotal role in ethylene biosynthesis were found to be down-regulated in the transgenic lines, thus suggesting deregulated ethylene accumulation in these plants. Overall, these results point to a role of TCTP in photosynthesis and hormone signaling.
番茄TCTP的过表达影响植物生物量的产生以及在胁迫下的表现。这些表型改变与主要与光合作用、脂肪酸代谢和水分运输相关的基因上调有关。翻译控制肿瘤蛋白（TCTP）是一种多面且高度保守的真核蛋白。在植物中，尽管有功能数据表明该蛋白参与细胞增殖和生长，但许多植物TCTP的详细生理作用仍知之甚少。在这里，我们聚焦于番茄中一个尚未被表征的TCTP（SlTCTP）。我们发现，当在烟草中过表达时，SlTCTP可能促进植物生物量的产生，并影响其在盐胁迫和渗透胁迫下的表现。对转基因植物的转录组分析揭示了主要与光合作用、脂肪酸代谢和水分运输相关的基因上调。这种诱导的光合基因表达与转基因植物光合速率和气孔导度的增加同时出现。此外，还检测到参与ABA介导的气孔运动调控的基因的转录调节。另一方面，发现乙烯生物合成中起关键作用的基因在转基因株系中下调，从而表明这些植物中乙烯积累失调。总体而言，这些结果表明TCTP在光合作用和激素信号传导中发挥作用。
