来自弗瑞德白血病细胞新复制染色质中核小体间距的改变。
Altered nucleosome spacing in newly replicated chromatin from Friend leukemia cells.
作者信息
Murphy R F, Wallace R B, Bonner J
出版信息
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5903-7. doi: 10.1073/pnas.75.12.5903.
Abstract
Chromatin from Friend leukemia cells labeled with [14C]thymidine for 24 hr followed by [3H]thymidine for 10 min is converted into nucleosomes by staphylococcal nuclease at only half the rate that total chromatin is converted. Polyacrylamide gel electrophoresis of nucleosomal DNA from cells labeled for 24 hr with [14C]thymidine followed by 10 min with [3H]thymidine demonstrates that the internucleosomal spacer of newly replicated chromatin is approximately 20 base pairs shorter than that of total chromatin. The implications of this difference for models of chromatin structure are discussed.
摘要
用[14C]胸苷标记24小时，随后用[3H]胸苷标记10分钟的弗瑞德白血病细胞染色质，经葡萄球菌核酸酶处理转化为核小体的速率仅为总染色质转化速率的一半。对先用[14C]胸苷标记24小时，随后用[3H]胸苷标记10分钟的细胞的核小体DNA进行聚丙烯酰胺凝胶电泳，结果表明新复制染色质的核小体间间隔比总染色质的核小体间间隔短约20个碱基对。本文讨论了这种差异对染色质结构模型的影响。
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