Morris N R
Cell. 1976 Dec;9(4 Pt 1):627-32. doi: 10.1016/0092-8674(76)90045-3.
The structure of chicken erythrocyte and chicken liver chromatin has been studied by enzymatic digestion with micrococcal nuclease and DNAase I. Comparison of the fragments produced by brief digestion with micrococcal nuclease indicates that chicken erythrocyte chromatin has a longer nucleosome repeat than chicken liver chromatin, 212 base pairs as opposed to 200 base pairs (bp). More extensive digestion with micrococcal nuclease demonstrates that both tissues have 140 bp mucleosome cores. The difference in the length of the nucleosome repeat must therefore result from a difference in the length of the DNA linker between cores. Chicken erythrocyte chromatin is known to contain a tissue-specific, H1-like histone, H5, and to be genetically dormant. A model is presented to explain how changes in histone H1 could cause changes in the nucleosome repeat length and thereby alter the genetic activity of chromatin.
通过用微球菌核酸酶和DNA酶I进行酶消化，对鸡红细胞和鸡肝染色质的结构进行了研究。用微球菌核酸酶进行短暂消化所产生的片段比较表明，鸡红细胞染色质的核小体重复长度比鸡肝染色质长，分别为212个碱基对和200个碱基对（bp）。用微球菌核酸酶进行更广泛的消化表明，两种组织都有140bp的核小体核心。因此，核小体重复长度的差异必定是由核心之间DNA连接体长度的差异导致的。已知鸡红细胞染色质含有一种组织特异性的、H1样组蛋白H5，并且处于基因休眠状态。提出了一个模型来解释组蛋白H1的变化如何导致核小体重复长度的变化，从而改变染色质的基因活性。
