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1
Computer programs for analysis of nucleic acid hybridization, thermal denaturation, and gel electrophoresis data.用于分析核酸杂交、热变性和凝胶电泳数据的计算机程序。
Nucleic Acids Res. 1979 Aug 24;6(12):3911-21. doi: 10.1093/nar/6.12.3911.
2
Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.通过凝胶分级分离并转移至重氮苄氧基甲基纸上检测特定RNA或DNA特定片段。
Methods Enzymol. 1979;68:220-42. doi: 10.1016/0076-6879(79)68017-5.
3
Computer programs to assist in high resolution thermal denaturation and circular dichroism studies on nucleic acids.用于辅助核酸高分辨率热变性和圆二色性研究的计算机程序。
Nucleic Acids Res. 1981 Dec 21;9(24):7033-40. doi: 10.1093/nar/9.24.7033.
4
Two-dimensional strandness-dependent electrophoresis: a method to characterize single-stranded DNA, double-stranded DNA, and RNA-DNA hybrids in complex samples.二维链依赖性电泳:一种表征复杂样品中单链DNA、双链DNA和RNA-DNA杂交体的方法。
Anal Biochem. 2006 Mar 1;350(1):120-7. doi: 10.1016/j.ab.2005.12.013. Epub 2006 Jan 17.
5
Improved Denaturation of Small RNA Duplexes and Its Application for Northern Blotting.小RNA双链体变性的改进及其在Northern印迹中的应用
Methods Mol Biol. 2017;1580:1-6. doi: 10.1007/978-1-4939-6866-4_1.
6
Denaturing polyacrylamide gel electrophoresis.变性聚丙烯酰胺凝胶电泳
Curr Protoc Hum Genet. 2001 May;Appendix 3:Appendix 3F. doi: 10.1002/0471142905.hga03fs00.
7
Analysis of DNA hybridization in polyacrylamide gel.聚丙烯酰胺凝胶中DNA杂交分析
Nucleic Acids Res Suppl. 2001(1):119-20. doi: 10.1093/nass/1.1.119.
8
Isolation of plant mitochondrial RNA.植物线粒体RNA的分离
Methods Enzymol. 1986;118:488-96. doi: 10.1016/0076-6879(86)18095-5.
9
Fractionation of low molecular weight DNA or RNA in polyacrylamide gels containing 98% formamide or 7 M urea.在含有98%甲酰胺或7M尿素的聚丙烯酰胺凝胶中对低分子量DNA或RNA进行分级分离。
Methods Enzymol. 1980;65(1):299-305. doi: 10.1016/s0076-6879(80)65040-x.
10
Improved conditions for in situ RNA-DNA hybridization.原位RNA-DNA杂交的改良条件。
FEBS Lett. 1973 Apr 1;31(1):85-8. doi: 10.1016/0014-5793(73)80078-x.

引用本文的文献

1
DNA hybridization analyses of a Gossypium allotetmploid and two closely related diploid species.棉花异源四倍体和两个近缘二倍体种的 DNA 杂交分析。
Theor Appl Genet. 1989 Apr;77(4):553-9. doi: 10.1007/BF00274278.
2
Oligosaccharides on living human neuroblastoma cells of dissimilar degrees of differentiation. A flow-cytometric study with sugar-specific lectins and glycosidases.不同分化程度的活体人类神经母细胞瘤细胞上的寡糖。一项使用糖特异性凝集素和糖苷酶的流式细胞术研究。
Cell Biophys. 1993 Aug-Dec;23(1-3):165-85. doi: 10.1007/BF02796511.
3
Isolation of newly replicated chromatin by using shallow metrizamide gradients.利用浅度Nycodenz梯度分离新复制的染色质。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3336-40. doi: 10.1073/pnas.77.6.3336.
4
Effect of estrogen on Xenopus laevis albumin mRNA levels.雌激素对非洲爪蟾白蛋白mRNA水平的影响。
Mol Cell Biochem. 1984 Sep;63(2):143-8. doi: 10.1007/BF00285222.
5
Nonlytic simian virus 40-specific 100K phosphoprotein is associated with anchorage-independent growth in simian virus 40-transformed and revertant mouse cell lines.非裂解性猿猴病毒40特异性100K磷蛋白与猿猴病毒40转化及回复的小鼠细胞系中的不依赖贴壁生长有关。
Mol Cell Biol. 1981 Nov;1(11):994-1006. doi: 10.1128/mcb.1.11.994-1006.1981.
6
The effect of specific glycosidases on Ricinus communis agglutinin binding to cell surfaces of two tumor sublines. A comparative flow-cytometric study.特定糖苷酶对蓖麻凝集素与两种肿瘤亚系细胞表面结合的影响。一项流式细胞术比较研究。
Cell Biophys. 1984 Sep;6(3):171-81. doi: 10.1007/BF02788617.
7
No evidence for post-transcriptional control of albumin and alpha-fetoprotein gene expression in developing rat liver neoplasia.在发育中的大鼠肝脏肿瘤形成过程中,没有证据表明白蛋白和甲胎蛋白基因表达存在转录后调控。
Nucleic Acids Res. 1982 Mar 25;10(6):1895-911. doi: 10.1093/nar/10.6.1895.
8
Processing of mammalian tRNA transcripts in vitro: different pre-tRNAs are processed along alternative pathways that contain a common rate-limiting step.体外加工哺乳动物tRNA转录本:不同的前体tRNA沿着包含共同限速步骤的替代途径进行加工。
Nucleic Acids Res. 1986 Jun 25;14(12):4849-64. doi: 10.1093/nar/14.12.4849.

本文引用的文献

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Theory of the effects of concentration and chain length on helix-coil equilibria in two-stranded nucleic acids.双链核酸中浓度和链长对螺旋-线圈平衡影响的理论
J Chem Phys. 1963 Nov 15;39(10):2719-21. doi: 10.1063/1.1734089.
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Stoichiometry of chromatin proteins.染色质蛋白的化学计量学。
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Studies on nucleic acid reassociation kinetics: reactivity of single-stranded tails in DNA-DNA renaturation.核酸复性动力学研究:DNA-DNA复性中单链尾的反应活性
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Structure of transcriptionally active chromatin.转录活性染色质的结构。
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A program for least squares analysis of reassociation and hybridization data.重缔合和杂交数据的最小二乘法分析程序。
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DNA sequencing and melting curve.DNA测序与熔解曲线。
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Altered nucleosome spacing in newly replicated chromatin from Friend leukemia cells.来自弗瑞德白血病细胞新复制染色质中核小体间距的改变。
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Chromatin structure in the cellular slime mold Dictyostelium discoideum.细胞黏菌盘基网柄菌中的染色质结构。
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Localization of the globin gene in the template active fraction of chromatin of Friend leukemia cells.珠蛋白基因在弗氏白血病细胞染色质模板活性组分中的定位。
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用于分析核酸杂交、热变性和凝胶电泳数据的计算机程序。

Computer programs for analysis of nucleic acid hybridization, thermal denaturation, and gel electrophoresis data.

作者信息

Murphy R F, Pearson W R, Bonner J

出版信息

Nucleic Acids Res. 1979 Aug 24;6(12):3911-21. doi: 10.1093/nar/6.12.3911.

DOI:10.1093/nar/6.12.3911
PMID:493129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327986/
Abstract

Computer programs for the analysis of data from techniques frequently used in nucleic acids research are described. In addition to calculating non-linear, least-squares solutions to equations describing these systems, the programs allow for data editing, normalization, plotting and storage, and are flexible and simple to use. Typical applications of the programs are described.

摘要

本文描述了用于分析核酸研究中常用技术所产生数据的计算机程序。除了计算描述这些系统的方程的非线性最小二乘解之外,这些程序还允许进行数据编辑、归一化、绘图和存储,并且灵活易用。文中还介绍了这些程序的典型应用。